Genotyping of Fasciola hepatica Isolated from Domestic Animals in the West of Iran

Author(s): Sahar Shokouhi, Jahangir Abdi*, Reza Valizadeh.

Journal Name: Infectious Disorders - Drug Targets
(Formerly Current Drug Targets - Infectious Disorders)

Volume 19 , Issue 4 , 2019

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Graphical Abstract:


Objectives: The most common parasites responsible for fascioliasis are F. hepatica and F. gigantica. Fascioliasis is an important problem in terms of health and economics. The western part of Iran is one of the major hubs of animal husbandry and the prevalence of fascioliasis is high. The aim of the current study was to determine the Fasciola spp. present in the western part of Iran. In the present study, 45 samples were collected from slaughterhouses in three provinces in the western part of Iran, including Ilam, Lorestan and Kermanshah.

Methods: The flukes were detected using morphological methods and the DNA of all samples was extracted. The ribosomal internal transcribed spacer (ITS1) was identified by PCR and PCR-RFLP techniques using the Rsa1 restriction enzyme. Then, 15% of the samples were sequenced.

Results: Based on their ITS1 sequence, all samples showed 700 bp bands. The results of sequencing showed a similarity of 99% to 100% across samples. Using the Rsa1 restriction enzyme, all samples produced three distinct bands (60 bp, 100 bp, and 360 bp). These results demonstrated that the most common fasciola in the western part of Iran in sheep and cattle is F. hepatica.

Conclusion: The results of the present study showed that only the species F. hepatica is parasitizing livestock in the western part of Iran. Further studies using new molecular markers for more accurate identification of fasciola-causing species will be useful in the control and prevention of fascioliasis.

Keywords: Fasciola hepatica, ITS1, PCR-RFLP, genotyping, F. gigantica, fascioliasis.

Ahmadi, N.A.; Meshkehkar, M. Prevalence and long term trend of liver fluke infections in sheep, goats and cattle slaughtered in Khuzestan, southwestern Iran. J. Paramed. Sci., 2010, 1(2)
Abdi, J.; Karimi, S.; Naserifar, R. Zoonotic parasitic diseases in Ilam Province, Western Iran. Afr. J. Microbiol. Res., 2013, 7(23), 2957-2960.
Beck, M.A.; Goater, C.P.; Colwell, D.D. Comparative recruitment, morphology and reproduction of a generalist trematode, Dicrocoelium dendriticum, in three species of host. Parasitology, 2015, 142(10), 1297-1305.
[] [PMID: 26059630]
Ashrafi, K.; Bargues, M.D.; O’Neill, S.; Mas-Coma, S. Fascioliasis: a worldwide parasitic disease of importance in travel medicine. Travel Med. Infect. Dis., 2014, 12(6 Pt A), 636-649.
[] [PMID: 25287722]
Cengiz, Z.T.; Yilmaz, H.; Dulger, A.C.; Cicek, M. Human infection with Dicrocoelium dendriticum in Turkey. Ann. Saudi Med., 2010, 30(2), 159-161.
[] [PMID: 20220269]
Díaz, P.; Paz-Silva, A.; Sánchez-Andrade, R.; Suárez, J.L.; Pedreira, J.; Arias, M.; Díez-Baños, P.; Morrondo, P. Assessment of climatic and orographic conditions on the infection by Calicophoron daubneyi and Dicrocoelium dendriticum in grazing beef cattle (NW Spain). Vet. Parasitol., 2007, 149(3-4), 285-289.
[] [PMID: 17888577]
Ezatpour, B.; Hasanvand, A.; Azami, M.; Anbari, K.; Ahmadpour, F. Prevalence of liver fluke infections in slaughtered animals in Lorestan, Iran. J. Parasit. Dis., 2015, 39(4), 725-729.
[] [PMID: 26688642]
Bernal, D.; Trelis, M.; Montaner, S.; Cantalapiedra, F.; Galiano, A.; Hackenberg, M.; Marcilla, A. Surface analysis of Dicrocoelium dendriticum. The molecular characterization of exosomes reveals the presence of miRNAs. J. Proteomics, 2014, 105, 232-241.
[] [PMID: 24561797]
Aleixo, M.A. Fasciola hepatica: epidemiology, perspectives in the diagnostic and the use of geoprocessing systems for prevalence studies. Semin. Cienc. Agrar., 2015, 36(3)
Cabeza-Barrera, I.; Cabezas-Fernández, T.; Salas Coronas, J.; Vázquez Villegas, J.; Cobo, F. Dicrocoelium dendriticum: an emerging spurious infection in a geographic area with a high level of immigration. Ann. Trop. Med. Parasitol., 2011, 105(5), 403-406.
[] [PMID: 21929882]
Ashrafi, K.; Saadat, F.; O’Neill, S.; Rahmati, B.; Amin Tahmasbi, H.; Pius Dalton, J.; Nadim, A.; Asadinezhad, M.; Rezvani, S.M. The endemicity of human fascioliasis in Guilan province, northern Iran: the baseline for implementation of control strategies. Iran. J. Public Health, 2015, 44(4), 501-511.
[PMID: 26056669]
Chang, Q-C.; Liu, G.H.; Gao, J.F.; Zheng, X.; Zhang, Y.; Duan, H.; Yue, D.M.; Fu, X.; Su, X.; Gao, Y.; Wang, C.R. Sequencing and characterization of the complete mitochondrial genome from the pancreatic fluke Eurytrema pancreaticum (Trematoda: Dicrocoeliidae). Gene, 2016, 576(1 Pt 1), 160-165.
[] [PMID: 26434796]
Ella, O.H.; Mohammad, A.E. Dicrocoelium dendriticum infection in a patient with chronic schistosomiasis haematobium. J. Egypt. Soc. Parasitol., 2015, 45(3), 629-632.
[] [PMID: 26939241]
Otranto, D.; Rehbein, S.; Weigl, S.; Cantacessi, C.; Parisi, A.; Lia, R.P.; Olson, P.D. Morphological and molecular differentiation between Dicrocoelium dendriticum (Rudolphi, 1819) and Dicrocoelium chinensis (Sudarikov and Ryjikov, 1951) Tang and Tang, 1978 (Platyhelminthes: Digenea). Acta Trop., 2007, 104(2-3), 91-98.
[] [PMID: 17803950]
Maurelli, M.P.; Rinaldi, L.; Capuano, F.; Perugini, A.G.; Veneziano, V.; Cringoli, G. Characterization of the 28S and the second internal transcribed spacer of ribosomal DNA of Dicrocoelium dendriticum and Dicrocoelium hospes. Parasitol. Res., 2007, 101(5), 1251-1255.
[] [PMID: 17605007]
Peng, M.; Ichinomiya, M.; Ohtori, M.; Ichikawa, M.; Shibahara, T.; Itagaki, T. Molecular characterization of Fasciola hepatica, Fasciola gigantica, and aspermic Fasciola sp. in China based on nuclear and mitochondrial DNA. Parasitol. Res., 2009, 105(3), 809-815.
[] [PMID: 19424719]
Lin, R.Q.; Dong, S.J.; Nie, K.; Wang, C.R.; Song, H.Q.; Li, A.X.; Huang, W.Y.; Zhu, X.Q. Sequence analysis of the first internal transcribed spacer of rDNA supports the existence of the intermediate Fasciola between F. hepatica and F. gigantica in mainland China. Parasitol. Res., 2007, 101(3), 813-817.
[] [PMID: 17356892]
Ai, L.; Chen, M.X.; Alasaad, S.; Elsheikha, H.M.; Li, J.; Li, H.L.; Lin, R.Q.; Zou, F.C.; Zhu, X.Q.; Chen, J.X. Genetic characterization, species differentiation and detection of Fasciola spp. by molecular approaches. Parasit. Vectors, 2011, 4(1), 101.
[] [PMID: 21658284]
Alasaad, S.; Soriguer, R.C.; Abu-Madi, M.; El Behairy, A.; Jowers, M.J.; Baños, P.D.; Píriz, A.; Fickel, J.; Zhu, X.Q. A TaqMan real-time PCR-based assay for the identification of Fasciola spp. Vet. Parasitol., 2011, 179(1-3), 266-271.
[] [PMID: 21334813]
Itagaki, T.; Kikawa, M.; Terasaki, K.; Shibahara, T.; Fukuda, K. Molecular characterization of parthenogenic Fasciola sp. in Korea on the basis of DNA sequences of ribosomal ITS1 and mitochondrial NDI gene. J. Vet. Med. Sci., 2005, 67(11), 1115-1118.
[] [PMID: 16327222]
Rokni, M.B.; Mirhendi, H.; Mizani, A.; Mohebali, M.; Sharbatkhori, M.; Kia, E.B.; Abdoli, H.; Izadi, S. Identification and differentiation of Fasciola hepatica and Fasciola gigantica using a simple PCR-restriction enzyme method. Exp. Parasitol., 2010, 124(2), 209-213.
[] [PMID: 19769969]
Ghavami, M. Genotypic and phenotypic analysis of Fasciola isolates. Iran. J. Parasitol., 2009, 4(3), 61-70.
Karimi, A. Genetic diagnosis of Fasciola species based on 18S ribosomal DNA sequences. J. Biol. Sci., 2008, 8(7), 1166-1173.

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Article Details

Year: 2019
Page: [409 - 413]
Pages: 5
DOI: 10.2174/1871526518666180806121934
Price: $65

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