Introduction: The majority of analytical methods used currently to quantify Vitamin B6 are
time-consuming, costly, non-environment friendly and destructive with respect to the analyzed material.
Objective: The objective of this study was to elaborate a novel method of vitamin B6 quantification in
commercial pharmaceutical preparations using Near Infrared Spectroscopy (NIRS).
Methods: Powdered tablets and calibration samples containing pyridoxine hydrochloride and microcrystalline
cellulose or lactose were employed. The raw NIRS spectra of calibration samples and
tablets were transformed by Multiplicative Scatter Correction (MSC) or by second derivative normalization
and used to generate the appropriate calibration plots. Alternatively, determination of pyridoxine
hydrochloride was performed by Principle Component Regression (PCR) for powdered tablets
and calibration sets of samples. The relationship was established between the Principal Component
PC1 calculated for the selected ranges of pretreated NIRS reflectance spectra of calibration standards
and the contents of pyridoxine hydrochloride.
Results: The samples of 3 different preparations containing 10 or 50 mg of pyridoxine hydrochloride
that differed in composition of the excipients were analyzed. Depending on the composition of the
formulation used and the selected wavelength range, the best recoveries for tested preparations were
Conclusion: In this study we have proved that quantification of hydrochloride pyridoxine in solid
pharmaceutical preparations by Near Infrared Reflectance Spectroscopy may be achieved with high
precision and accuracy. Our method is cheap, rapid, environmentally-friendly and may be used for
routine analyzes in the Quality Control laboratory.