New Avenues in Drug Discovery and Bioactive Natural Products

Screening of Natural Resources Through High Performance Liquid Chromatography

Author(s): Ganesh Prasad Mishra, Manish Pathak, Sokindra Kumar, Lubhan Singh, Rupesh Kumar Pandey, Prasanjit Paul, Bina Gidwani, Ravindra Kumar Pandey and Shiv Shankar Shukla * .

Pp: 273-281 (9)

DOI: 10.2174/9789815136326123020014

* (Excluding Mailing and Handling)


Currently, analytical techniques play a crucial role in drug discoveries and isolation processes. Different advanced methods are available for screening natural resources into desirable forms. Among them, chromatographic techniques are the most reliable and applicable for small quantities of samples. Chromatography is a technique for separating mixtures that depend upon differential affinities of solutes between two immiscible phases. One of the phases is a fixed bed of a large surface area while other fluid flows through or over a fixed phase. The greatest utility of chromatography lies in its ability to separate mixtures of solutes so that several individual substances may be quantities isolated in a pure state. Separation and identification of a natural substance can be challenging. Natural substances are a mixture of different components with different physiochemical properties. They are available as natural sources in large to small quantities in the form of a mixture. Herein, we are trying to explain different types of chromatographic techniques and types of strategies adopted to identify and isolate natural substances.

Keywords: Analytical techniques, Chromatographic column, Elution, Natural substances, Retention time.

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