Recent Advances in Biotechnology

Volume: 5

Advances in the Human Preimplantation Embryonic Culture System

Author(s): Manar M. Hozyen* and Islam M. Saadeldin * .

Pp: 153-173 (21)

DOI: 10.2174/9789815051667122050009

* (Excluding Mailing and Handling)


The embryonic culture system is the main part of in-vitro embryonic development. The culture system consists of chemical factors such as the chemical composition of the culture media and physical factors such as temperature, pH, oil overlay and, incubation systems. The optimal culture conditions are needed to support embryonic development in-vitro, increase pregnancy, implantation rates, and decrease the level of pregnancy loss. Any suboptimality in culture conditions may impair embryonic development and affect subsequent viability. In this chapter, we will focus on the current culture system in the field of assisted reproductive technology (ART), passing by current culture media formulations, deficiencies, culture devices used, and the areas that need further investigations. 

Keywords: Culture dishes, Culture media, Culture system, Embryo culture, Oil overlay, pH.

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