Generic placeholder image

Anti-Cancer Agents in Medicinal Chemistry


ISSN (Print): 1871-5206
ISSN (Online): 1875-5992

Research Article

Contribution of Major Polyphenols to the Antioxidant Profile and Cytotoxic Activity of Olive Leaves

Author(s): Zouhaier Bouallagui*, Asma Mahmoudi, Amina Maalej, Fatma Hadrich, Hiroko Isoda and Sami Sayadi

Volume 19 , Issue 13 , 2019

Page: [1651 - 1657] Pages: 7

DOI: 10.2174/1871520619666190416101622

Price: $65


Aims: This study was designed to investigate the phytochemical profile and the cytotoxic activities of the eco-friendly extracts of olive leaves from Chemlali cultivar.

Materials and Methods: The Phenolic composition of olive leaves extracts, the antioxidant activity and the cytotoxic effects against MCF-7 and HepG2 cells were determined.

Results: Olive leaves extracts showed relevant total polyphenols contents. Oleuropein was the major detected phenolic compound reaching a concentration of 16.9 mg/ml. The antioxidant potential of the studied extracts varied from 23.7 to 46.5mM Trolox equivalents as revealed by DPPH and ABTS assays. Cytotoxicity experiments showed similar trends for both HepG2 and MCF-7 cells with the infusion extract being the most active.

Conclusion: This study denotes that olive leaves may have great potential as endless bioresource of valuable bioactive compounds which may have a wide application.

Keywords: Olea europaea, “Chemlali” cultivar, leaves, phytochemical profile, bioactivities, polyphenols.

Graphical Abstract
Abaza, L.; Talorete, T.P.N.; Yamada, P.; Kurita, Y.; Zarrouk, M.; Isoda, H. Induction of growth inhibition and differentiation of human leukemia HL-60 cells by a Tunisian gerboui olive leaf extract. Biosci. Biotechnol. Biochem., 2007, 71(5), 1306-1312.
Lipworth, L.; Martinez, M.E.; Angell, J.; Hsieh, C.C.; Trichopoulos, D. Olive oil and human cancer: An assessment of the evidence. Prev. Med., 1997, 26(2), 181-190.
Martin-Pelaez, S.; Covas, M.I.; Fito, M.; Kusar, A.; Pravst, I. Health effects of olive oil polyphenols: Recent advances and possibilities for the use of health claims. Mol. Nutr. Food Res., 2013, 57(5), 760-771.
Kchaou, H.; Larbi, A.; Gargouri, K.; Chaieb, M.; Morales, F.; Msallem, M. Assessment of tolerance to NaCl salinity of five olive cultivars, based on growth characteristics and Na+ and Cl- exclusion mechanisms. Sci. Hortic., 2010, 124(3), 306-315.
Garcia, M.A.I.; Moumen, A.; Ruiz, D.R.Y.; Alcaide, E.M. Chemical composition and nutrients availability for goats and sheep of two-stage olive cake and olive leaves. Anim. Feed Sci. Technol., 2003, 107(1-4), 61-74.
Soler-Rivas, C.; Espin, J.C.; Wichers, H.J. Oleuropein and related compounds. J. Sci. Food Agric., 2000, 80(7), 1013-1023.
Benavente-Garcia, O.; Castillo, J.; Lorente, J.; Ortuno, A.; Del Rio, J.A. Antioxidant activity of phenolics extracted from Olea europaea L-leaves. Food Chem., 2000, 68(4), 457-462.
El, S.N.; Karakaya, S. Olive tree (Olea europaea) leaves: Potential beneficial effects on human health. Nutr. Rev., 2009, 67(11), 632-638.
Ghanbari, R.; Anwar, F.; Alkharfy, K.M.; Gilani, A.H.; Saari, N. Valuable nutrients and functional bioactives in different parts of olive (Olea europaea L.)-A review. Int. J. Mol. Sci., 2012, 13(3), 3291-3340.
Lee-Huang, S.; Zhang, L.; Huang, P.L.; Chang, Y.T.; Huang, P.L. Anti-HIV activity of olive leaf extract (OLE) and modulation of host cell gene expression by HIV-1 infection and OLE treatment. Biochem. Biophys. Res. Commun., 2003, 307(4), 1029-1037.
Markin, D.; Duek, L.; Berdicevsky, I. In vitro antimicrobial activity of olive leaves. Mycoses, 2003, 46(3-4), 132-136.
Bhattacharya, A.; Sood, P.; Citovsky, V. The roles of plant phenolics in defence and communication during Agrobacterium and Rhizobium infection. Mol. Plant Pathol., 2010, 11(5), 705-719.
Servili, M.; Montedoro, G. Contribution of phenolic compounds to virgin olive oil quality. Eur. J. Lipid Sci. Technol., 2002, 104(9-10), 602-613.
Brahmi, F.; Mechri, B.; Dhibi, M.; Hammami, M. Variations in phenolic compounds and antiradical scavenging activity of Olea europaea leaves and fruits extracts collected in two different seasons. Ind. Crops Prod., 2013, 49, 256-264.
Veneziani, G.; Esposto, S.; Taticchi, A.; Urbani, S.; Selvaggini, R.; Sordini, B.; Servili, M. Characterization of phenolic and volatile composition of extra virgin olive oil extracted from six Italian cultivars using a cooling treatment of olive paste. LWT - Food Sci. Technol., 2018, 87, 523-528.
Singleton, V.L.; Rossi, J.A. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticulture., 1965, 16(3), 144-158.
Quettier-Deleu, C.; Gressier, B.; Vasseur, J.; Dine, T.; Brunet, C.; Luyckx, M.; Cazin, M.; Cazin, J.C.; Bailleul, F.; Trotin, F. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J. Ethnopharmacol., 2000, 72(1-2), 35-42.
Brand-Williams, W.; Cuvelier, M.E.; Berset, C. Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci. Technol., 1995, 28(1), 25-30.
Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med., 1999, 26(9-10), 1231-1237.
Herrero, M.; Temirzoda, T.N.; Segura-Carretero, A.; Quirantes, R.; Plaza, M.; Ibanez, E. New possibilities for the valorization of olive oil by-products. J. Chromatogr. A, 2011, 1218(42), 7511-7520.
Souilem, S.; Fki, I.; Kobayashi, I.; Khalid, N.; Neves, M.A.; Isoda, H.; Sayadi, S.; Nakajima, M. Emerging technologies for recovery of value-added components from olive leaves and their applications in food/feed industries. Food Bioprocess Technol., 2017, 10(2), 229-248.
Abaza, L.; Taamalli, A.; Nsir, H.; Zarrouk, M. Olive Tree (Olea europeae L.) leaves: Importance and advances in the analysis of phenolic compounds. Antioxidants (Basel), 2015, 4(4), 682-698.
Rahmanian, N.; Jafari, S.M.; Wani, T.A. Bioactive profile, dehydration, extraction and application of the bioactive components of olive leaves. Trends Food Sci. Technol., 2015, 42(2), 150-172.
Nenadis, N.; Kyriakoudi, A.; Tsimidou, M.Z. Impact of alkaline or acid digestion to antioxidant activity, phenolic content and composition of rice hull extracts. LWT - Food Sci. Technol., 2013, 54(1), 207-215.
Kiritsakis, K.; Kontominas, M.G.; Kontogiorgis, C.; Hadjipavlou-Litina, D.; Moustakas, A.; Kiritsakis, A. Composition and antioxidant activity of olive leaf extracts from greek olive cultivars. J. Am. Oil Chemists. Soc., 2010, 87(4), 369-376.
Floegel, A.; Kim, D-O.; Chung, S-J.; Koo, S.I.; Chun, O.K. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J. Food Composit. Anal., 2011, 24(7), 1043-1048.
Surh, Y.J. Cancer chemoprevention with dietary phytochemicals. Nat. Rev. Cancer, 2003, 3(10), 768-780.
Han, J.; Talorete, T.P.N.; Yamada, P.; Isoda, H. Anti-proliferative and apoptotic effects of oleuropein and hydroxytyrosol on human breast cancer MCF-7 cells. Cytotechnology, 2009, 59(1), 45-53.
Goulas, V.; Exarchou, V.; Troganis, A.N.; Psomiadou, E.; Fotsis, T.; Briasoulis, E.; Gerothanassis, I.P. Phytochemicals in olive-leaf extracts and their antiproliferative activity against cancer and endothelial cells. Mol. Nut. Food Res., 2009, 53(5), 600-608.
Boss, A.; Bishop, K.S.; Marlow, G.; Barnett, M.P.; Ferguson, L.R. Evidence to support the anti-cancer effect of olive leaf extract and future directions. Nutrients, 2016, 8(8)E513
Talorete, T.P.N.; Bouaziz, M.; Sayadi, S.; Isoda, H. Influence of medium type and serum on MTT reduction by flavonoids in the absence of cells. Cytotechnology, 2006, 52(3), 189-198.
Murkies, A.L.; Wilcox, G.; Davis, S.R. Clinical review 92: Phytoestrogens. J. Clin. Endocrinol. Metab., 1998, 83(2), 297-303.

Rights & Permissions Print Export Cite as
© 2022 Bentham Science Publishers | Privacy Policy