Background: Infectious diseases caused by various pathogens including bacteria, fungi, viruses, and parasites are a very common danger to people health.Objective: The main aim of the present study was to evaluate the in vitro pharmacological effects of Atropa accuminata and Atropa belladonna extracts through antibacterial and antioxidant activities to find basic information for the development of potential therapeutic drugs from these plants. Methods: In the current research, the antioxidant constituents of Atropa accuminata and Atropa belladonna were assessed through free scavenging methods and thin layer chromatography (TLC) followed by DPPH (2, 2-Diphenyl-1-picrylhydrazyl) spray technique. Antibacterial activity of the leaf and root extracts was analysed for nine bacteria through agar well diffusion method. The effect of standard antibiotics was also determined by the agar disc diffusion method. Total phenolic and total flavonoid contents were estimated by folin-ciocalteau and aluminum chloride methods. Results: Both medicinal plants exhibited low to high antibacterial activity against all tested bacterial pathogens. TLC-spot screening indicated the presence of antimicrobial agents. Phenolic contents were higher in ethanolic and DMSO extracts of A. belladonna roots compared to the leaf extracts whereas flavonoid contents were higher in the DMSO extracts of A. belladonna leaf. High-performance liquid chromatography indicated the presence of a variety of sugar and organic acids. Conclusion: Such findings would be useful in promoting research aiming at the discovery of new antimicrobial agents and antioxidants.