Proteomics and bioinformatics approach were applied for the analyzing of wheat leaf proteins composition and function. Wheat proteins were precipitated by ammonium sulfate and analyzed by two-dimensional gel electrophoresis and mass spectrometry. A total of 200 wheat proteins were selected to identify based on reproducibility and relative quantity, and 123 proteins were identified with an identification success rate of 61.5 ;. The classifications of these proteins by BGSSJ (bioinformatic software) were mainly classified by their molecular, biological and cellular function. Proteins grouped under the molecular function were involved in catalytic, binding and antioxidant activity. The catalytic activity of identified wheat proteins included oxidoreductase, transferase, hydrolase, lyase and isomerase. Only 10.6 % of the wheat protein identifications lacked ascertainable functions. These results provided the information to investigate the composition and function of proteins found in wheat leaf, and enhanced the feasibility of future research on wheat.