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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Hydroxyapatite Elution Behavior of Human Nucleotide Excision Repair Protein XPA and Fragments of XPA

Author(s): Garry W. Buchko and Michael A. Kennedy

Volume 8 , Issue 5 , 2001

Page: [357 - 365] Pages: 9

DOI: 10.2174/0929866013409300

Price: $65

Abstract

The hydroxyapatite elution behavior of 3 fragments of human XPA (M98-F219 = XPA-MBD M59-F219 = XPA-EM M59-M273 = XPA-deltaN58) and full-length human XPA containing a 6-residue, N-terminal histidine tag (h6-XPA) are described. With increasing concentration of potassium phosphate the proteins elute in the order: XPA-EM, XPA-MBD, XPA-deltaN58, and h6-XPA. If hydroxyapatite affinity is related to DNA-binding affinity, then h6-XPA and XPA-deltaN58 may bind DNA more tightly than XPA-MBD and XPA-EM.

Keywords: REPAIR PROTEIN XPA, Nucleotide excision repair (NER), XPA gene


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