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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Preparation, Crystallization and Preliminary X-Ray Analysis of Threonine Synthase from Streptococcus mutans

Author(s): De-Wei Tang, Lan-Fen Li, Ya-Mei Yu, Xiang-Yu Liu, Xiao-Dong Su, Xiaojun Zhao and Yu-He Liang

Volume 14 , Issue 8 , 2007

Page: [836 - 838] Pages: 3

DOI: 10.2174/092986607781483633

Price: $65

Abstract

The thrC gene of Streptococcus mutans encodes threonine synthase, which is a potential target for drug design. To study the structure and function of the enzyme, the thrC gene was amplified from Streptococcus mutans genomic DNA and cloned into the expression vector pET28α. The protein was expressed in Escherichia coli in soluble form and purified to homogeneity. Crystals suitable for X-ray diffraction were obtained by hanging-drop vapor diffusion method. The crystal diffracted to 2.5 Å and belonged to space group P31 or P32, with unit cell parameters a=b=60.39 Å, c=118.62 Å.

Keywords: pyridoxal 5-phosphate, X-ray diffraction data, polymerase chain reaction amplification, Protein Purification, Threonine synthase


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