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Current Drug Delivery


ISSN (Print): 1567-2018
ISSN (Online): 1875-5704

Research Article

Evaluating the Effect of Integra Seeded with Adipose Tissue-Derived Stem Cells or Fibroblasts in Wound Healing

Author(s): Yuchang Wang, Quanrui Feng, Zhanfei Li, Xiangjun Bai, Yiping Wu and Yukun Liu*

Volume 17 , Issue 7 , 2020

Page: [629 - 635] Pages: 7

DOI: 10.2174/1567201817666200512104004

Price: $65


Background: Extensive loss of skin in burn patients can have devastating consequences, both physically and mentally. Adipose-Derived Stem Cells (ADSCs) and fibroblasts are known to play significant roles in the process of wound healing. Recently, bioengineered skin has been considered for wound healing purposes.

Methods: Investigate the effect of Integra seeded with ADSCs, fibroblasts, or both on wound healing.

Results: We found that when Integra is seeded with ADSCs and fibroblasts, both types of cells incorporate and proliferate, the phenomenon becoming more robust when the cells are co-cultured on Integra, both in vitro and in vivo. In addition, when these cells are seeded on Integra, they stimulate epithelization with no signs of inflammation and skin necrosis being observed when transplanted on animals for 7 days.

Conclusion: ADSCs and fibroblasts seeded on Integra could decrease the number of α-SMA positive myofibroblasts, leading to scarless wound healing. The evidence from this study is strongly supportive that Integra seeded with ADSCs and fibroblasts is an appropriate and effective bioengineered skin for wound healing.

Keywords: Adipose tissue-derived stem cells, integra, fibroblasts, wound healing, bioengineered, infection.

Graphical Abstract
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