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Endocrine, Metabolic & Immune Disorders - Drug Targets

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Research Article

Modulation of Hematological Indices of Normal and Alloxan-Induced Diabetic Rabbits by Aqueous Extract of Pleurotus tuberregium Sclerotia

Author(s): Ifeanacho M. Onuekwuzu, Ikewuchi C. Chidinma* and Ikewuchi J. Chigozie

Volume 20, Issue 3, 2020

Page: [380 - 387] Pages: 8

DOI: 10.2174/1871530319666190809155506

Price: $65

Abstract

Objective: The ability of an aqueous extract of the sclerotia of Pleurotus tuberregium to modulate hematological parameters was investigated in normal and alloxan treated rabbits.

Methods: The extract was subjected to atomic absorption spectrophotometric and flame ionization detector-coupled-gas chromatographic (GC-FID) analysis. Diabetes mellitus was induced by a 120 mg/kg body weight intravenous injection of alloxan. Metformin was orally administered at 50 mg/kg, while the extract was administered (both to normal and diabetic rabbits) at 100, 200 and 300 mg/kg.

Results: Analysis of the extract showed that it had high contents of calcium, magnesium, manganese and potassium. Eleven known glycosides were detected, comprising mainly of amygdalin (37.7%), digoxin (14.4%), dhurrin (14.0%), linamarin (13.6%), prunasin (10.8%) and digitoxin (8.4%). Also detected were twelve known saponins, consisting mainly of sapogenin (40.3%) and neochlorogenin (21.8%); and twelve known lignans, consisting mainly of matairesinol (59.7%), secoisolariciresinol (20.9%) and lariciresinol (14.9%). Compared to the Diabetic control, the hematocrit, hemoglobin concentration, mean cell hemoglobin, mean cell hemoglobin concentration, mean corpuscular volume, red cell distribution width; and red cell, total white cell, lymphocytes, granulocytes and platelet counts of the treated groups were significantly (p<0.05) higher.

Conclusion: The above result showed that the extract had a positive effect on the hemopoietic system of the treated animals, at least at the doses at which it was administered in this study.

Keywords: Glycosides, lignans, platelet indices, Pleurotus tuberregium sclerotia, red cell indices, white cell indices.

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[1]
Gkrania-Klotsas, E.; Ye, Z.; Cooper, A.J.; Sharp, S.J.; Luben, R.; Biggs, M.L.; Chen, L.K.; Gokulakrishnan, K.; Hanefeld, M.; Ingelsson, E.; Lai, W.A.; Lin, S.Y.; Lind, L.; Lohsoonthorn, V.; Mohan, V.; Muscari, A.; Nilsson, G.; Ohrvik, J.; Chao , Qiang. J.; Jenny, N.S.; Tamakoshi, K.; Temelkova-Kurktschiev, T.; Wang, Y.Y.; Yajnik, C.S.; Zoli, M.; Khaw, K.T.; Forouhi, N.G.; Wareham, N.J.; Langenberg, C. Differential white blood cell count and type 2 diabetes: systematic review and meta-analysis of cross-sectional and prospective studies. PLoS One, 2010, 5(10) e13405
[http://dx.doi.org/10.1371/journal.pone.0013405] [PMID: 20976133]
[2]
Biadgo, B.; Melku, M.; Abebe, S.M.; Abebe, M. Hematological indices and their correlation with fasting blood glucose level and anthropometric measurements in type 2 diabetes mellitus patients in Gondar, Northwest Ethiopia. Diabetes Metab. Syndr. Obes., 2016, 9, 91-99.
[http://dx.doi.org/10.2147/DMSO.S97563] [PMID: 27042134]
[3]
Al Salhen, K.S.; Mahmoud, A.Y. Hematological profile of patients with type 2 diabetic mellitus in El-Beida, Libya. Ibnosina J. Med. Biomed. Sci, 2017, 9, 76-80.
[http://dx.doi.org/10.4103/1947-489X.210115]
[4]
Malandrino, N.; Wu, W.C.; Taveira, T.H.; Whitlatch, H.B.; Smith, R.J. Association between RDW width and macrovascular complications in diabetes. Diabetologica, 2012, 55, 226-235.
[http://dx.doi.org/10.1007/s00125-011-2331-1]
[5]
Moradi, S.; Kerman, S.R.J.; Rohani, F.; Salari, F. Association between diabetes complications and leukocyte counts in Iranian patients. J. Inflamm. Res., 2012, 5, 7-11.
[http://dx.doi.org/10.2147/JIR.S26917] [PMID: 22334791]
[6]
Thomas, M.C.; MacIsaac, R.J.; Tsalamandris, C.; Power, D.; Jerums, G. Unrecognised anaemia in patients with diabetes. Diabetes Care, 2003, 26, 1164-1169.
[http://dx.doi.org/10.2337/diacare.26.4.1164] [PMID: 12663591]
[7]
Thomas, M.C.; MacIsaac, R.J.; Tsalamandris, C.; Molyneaux, L.; Goubina, I.; Fulcher, G.; Yue, D.; Jerums, G. The burden of anaemia in type 2 diabetes and the role of nephropathy: a cross-sectional audit. Nephrol. Dial. Transplant., 2004, 19(7), 1792-1797.
[http://dx.doi.org/10.1093/ndt/gfh248] [PMID: 15102962]
[8]
Angelousi, A.; Larger, E. Anaemia, a common but often unrecognized risk in diabetic patients: a review. Diabetes Metab., 2015, 41(1), 18-27.
[http://dx.doi.org/10.1016/j.diabet.2014.06.001] [PMID: 25043174]
[9]
Antwi-Bafour, S.; Hammond, S.; Adjei, J.K.; Kyeremeh, R.; Martin-Odoom, A.; Ekem, I. A case-control study of prevalence of anemia among patients with type 2 diabetes. J. Med. Case Reports, 2016, 10(1), 110.
[http://dx.doi.org/10.1186/s13256-016-0889-4] [PMID: 27142617]
[10]
Barbieri, J.; Fontela, P.C.; Winkelmann, E.R.; Zimmermann, C.E.P.; Sandri, Y.P.; Mallet, E.K.V.; Frizzo, M.N. Anemia in patients with type 2 diabetes mellitus. Anemia, 2015, 2015354737
[http://dx.doi.org/10.1155/2015/354737] [PMID: 26640706]
[11]
McGill, J.B.; Bell, D.S. Anemia and the role of erythropoietin in diabetes. J. Diabetes Complications, 2006, 20(4), 262-272.
[http://dx.doi.org/10.1016/j.jdiacomp.2005.08.001] [PMID: 16798479]
[12]
Hamed, N.A.M. Alterations in hematological parameters: Could it be a marker in diabetes mellitus? BAOJ Diabetes, 2016, 2, 009.
[13]
Thomas, M.; Tsalamandris, C.; MacIsaac, R.; Jerums, G. Anaemia in diabetes: an emerging complication of microvascular disease. Curr. Diabetes Rev., 2005, 1(1), 107-126.
[http://dx.doi.org/10.2174/1573399052952587] [PMID: 18220587]
[14]
Astor, B.C.; Muntner, P.; Levin, A.; Eustace, J.A.; Coresh, J. Association of kidney function with anemia: the Third National Health and Nutrition Examination Survey (1988-1994). Arch. Intern. Med., 2002, 162(12), 1401-1408.
[http://dx.doi.org/10.1001/archinte.162.12.1401] [PMID: 12076240]
[15]
Chung, F-M.; Tsai, J.C-R.; Chang, D-M.; Shin, S-J.; Lee, Y-J. Peripheral total and differential leukocyte count in diabetic nephropathy: the relationship of plasma leptin to leukocytosis. Diabetes Care, 2005, 28(7), 1710-1717.
[http://dx.doi.org/10.2337/diacare.28.7.1710] [PMID: 15983324]
[16]
Demirtas, L.; Degirmenci, H.; Akbas, E.M.; Ozcicek, A.; Timuroglu, A.; Gurel, A.; Ozcicek, F. Association of hematological indicies with diabetes, impaired glucose regulation and microvascular complications of diabetes. Int. J. Clin. Exp. Med., 2015, 8(7), 11420-11427.
[PMID: 26379958]
[17]
Yilmaz, T.; Yilmaz, A. Relationship between altered platelet morphological parameters and retinopathy in patients with type 2 diabetes mellitus. J. Ophthalmol., 2016, 20169213623
[http://dx.doi.org/10.1155/2016/9213623] [PMID: 27190641]
[18]
Ikewuchi, C.C.; Ikewuchi, J.C. Chemical profile of Pleurotus tuberregium (Fr) Sing’s sclerotia. Pac. J. Sci. Technol., 2009, 10, 357-362.
[19]
Ikewuchi, J.C.; Ikewuchi, C.C. Nutrient composition of Pleurotus tuberregium (Fr) Sing’s sclerotia. GJPAS, 2011, 17, 51-54.
[20]
Ikewuchi, J.C.; Ikewuchi, C.C.; Ifeanacho, M.O.; Igboh, N.M.; Ijeh, I.I. Gas chromatography-flame ionization detector analysis of the phytochemical composition of Pleurotus tuberregium (Fr) Sing’s sclerotia: Potential benefits. Pac. J. Sci. Technol., 2013, 14, 342-359.
[21]
Ikewuchi, J.C.; Ikewuchi, C.C.; Ifeanacho, M.O.; Igboh, N.M.; Ijeh, I.I. Moderation of hematological and plasma biochemical indices of sub-chronic salt-loaded rats by aqueous extract of the sclerotia of Pleurotus tuberregium (Fr) Sing’s: implications for the reduction of cardiovascular risk. J. Ethnopharmacol., 2013, 150(2), 466-476.
[http://dx.doi.org/10.1016/j.jep.2013.09.002] [PMID: 24055467]
[22]
Ikewuchi, J.C.; Ikewuchi, C.C.; Ifeanacho, M.O.; Igboh, N.M. Blood pressure lowering activity of a flavonoid and phytosterol rich extract of the sclerotia of Pleurotus tuberregium (Fr) Sing’s in salt-loaded rats. Biomed. Prevent. Nutr., 2014, 4, 257-263.
[http://dx.doi.org/10.1016/j.bionut.2014.02.006]
[23]
Ikewuchi, C.C.; Ikewuchi, J.C.; Ifeanacho, M.O. Restoration of plasma markers of liver and kidney functions/integrity in alloxan-induced diabetic rabbits by aqueous extract of Pleurotus tuberregium sclerotia. Biomed. Pharmacother., 2017, 95, 1809-1814.
[http://dx.doi.org/10.1016/j.biopha.2017.09.075] [PMID: 28968925]
[24]
Onuekwuzu, I.M.; Chidinma, I.C.; Chigozie, I.J. Anti-diabetic effect of a flavonoid and sitosterol - rich aqueous extract of Pleurotus tuberregium sclerotia in alloxan-induced diabetic rabbits. Endocr. Metab. Immune Disord. Drug Targets, 2019, 19(8), 1148-1156.
[http://dx.doi.org/10.2174/1871530319666190206213843] [PMID: 30727935]
[25]
Chung, K.T.; Wong, T.Y.; Wei, C.I.; Huang, Y.W.; Lin, Y. Tannins and human health: a review. Crit. Rev. Food Sci. Nutr., 1998, 38(6), 421-464.
[http://dx.doi.org/10.1080/10408699891274273] [PMID: 9759559]
[26]
Sen, G.; Mandal, S.; Saha Roy, S.; Mukhopadhyay, S.; Biswas, T. Therapeutic use of quercetin in the control of infection and anemia associated with visceral leishmaniasis. Free Radic. Biol. Med., 2005, 38(9), 1257-1264.
[http://dx.doi.org/10.1016/j.freeradbiomed.2005.01.014] [PMID: 15808423]
[27]
European Food Safety Authority. Scientific Opinion of the Panel on Contaminants in the Food Chain on a request from the European Commission on Saponins in Madhuca longifolia L. as undesirable substances in animal feed. EFSA J., 2009, 979, 1-36.
[28]
Davidovi, V.; Lazarevi, M.; Joksimovi-Todorovi, M.; Maksimovi, Z.; Jovanovi, M. The effect of the extract of rhizome and root of hellebore (Helleborus odorus W. et K.) on parameters of white blood count and degree of phagocytosis in Wistar rats. Acta Vet. (Beogr.), 2010, 60, 605-618.
[http://dx.doi.org/10.2298/AVB1006605D]
[29]
Schoorl, M.; Schoorl, M.; van der Gaag, D.; Bartels, P.C.M. Effects of iron supplementation on red blood cell hemoglobin content in pregnancy. Hematol. Rep., 2012, 4(4)e24
[http://dx.doi.org/10.4081/hr.2012.e24] [PMID: 23355942]
[30]
Dogar, M.Z.; Latif, I.; Saba, A.; Kanwal, S.; Khan, A.H.; Khan, Z.I.; Ahmad, K. Evaluation of iron supplementation effects on various haematological parameters in pregnant anemic patients of Sargodha Region in Pakistan. J. Environ. Anal. Toxicol., 2013, 3, 179.
[31]
Othman, H.K.; Maulood, I.M.; Mahmud, A.M.R. Effects of potassium and magnesium on some hematological profiles in two kidney, one clip-hypertensive and normotensive rats. ZJPAS, 2016, 28, 19-32.
[32]
Motsara, M.R.; Roy, R.N. Guide to laboratory establishment for plant nutrient analysis. In: FAO Fertilizer and Plant Nutrition Bulletin 19; Food and Agri-culture Organization of the United Nations: Rome, 2008. ISBN 978-92-5-105981-4
[33]
Mushtaq, A.; Akbar, S.; Zargar, M.A.; Wali, A.F.; Malik, A.H.; Dar, M.Y.; Hamid, R.; Ganai, B.A. Phytochemical screening, physicochemical properties, acute toxicity testing and screening of hypoglycaemic activity of extracts of Eremurus himalaicus baker in normoglycaemic Wistar strain albino rats. BioMed Res. Int., 2014, 2014867547
[http://dx.doi.org/10.1155/2014/867547] [PMID: 24864262]
[34]
Evans, W.C. Alkaloids.Trease and Evans Pharmacognosy, 15th ed; Evans, W.C., Ed.; Elsevier: India, 2005, pp. 333-393.
[35]
Evans, W.C. Saponins, cardioactive drugs and other steroids.Trease and Evans Pharmacognosy, 15th ed; Evans, W.C., Ed.; Elsevier: India, 2005, pp. 289-314.
[36]
Gul, R.; Jan, S.U.; Faridullah, S.; Sherani, S.; Jahan, N. Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from Ephedra intermedia indigenous to Balochistan. ScientificWorldJournal, 2017, 20175873648
[http://dx.doi.org/10.1155/2017/5873648] [PMID: 28386582]
[37]
Guo, M.; Zhang, L.; Liu, Z. Analysis of saponins from leaves of Aralia elata by liquid chromatography and multi-stage tandem mass spectrometry. Anal. Sci., 2009, 25(6), 753-758.
[http://dx.doi.org/10.2116/analsci.25.753] [PMID: 19531882]
[38]
Oluwaniyi, O.O.; Ibiyemi, S.A. A study of the extractability of thevetia glycosides with alcohol mixture. J. Food Technol., 2007, 5, 147-151.
[39]
Chapman, J.M.; Knoy, C.; Kindscher, K.; Brown, R.C.D.; Niemann, S. Identification of antineoplastic and neurotrophic lignans in medicinal prairie plants by liquid chromatography electron impact mass spectrometry (LC/EI/MS). eprint of Poster from Kansas City Life Sciences Day 2006, 2006.http://www.cssco.com/files/KC%20Life%20Science%202006.pdf
[40]
FAO. Protein quality evaluation: Report of Joint FAO/WHO Expert Consultation. FAO Food and Nutrition Paper 51; Food and Agriculture Organization of the United Nations: Rome, 1991.
[41]
Ramin, N.; Thieme, R.; Fischer, S.; Schindler, M.; Schmidt, T.; Fischer, B.; Navarrete Santos, A. Maternal diabetes impairs gastrulation and insulin and IGF-I receptor expression in rabbit blastocysts. Endocrinology, 2010, 151(9), 4158-4167.
[http://dx.doi.org/10.1210/en.2010-0187] [PMID: 20631000]
[42]
De Franceschi, L.; Brugnara, C.; Beuzard, Y. Dietary magnesium supplementation ameliorates anemia in a mouse model of β-thalassemia. Blood, 1997, 90(3), 1283-1290.
[http://dx.doi.org/10.1182/blood.V90.3.1283] [PMID: 9242563]
[43]
Cinar, V.; Nizamlioglu, M.; Mogulkoc, R.; Baltaci, A.K. Effects of magnesium supplementation on blood parameters of athletes at rest and after exercise. Biol. Trace Elem. Res., 2007, 115(3), 205-212.
[http://dx.doi.org/10.1007/BF02685995] [PMID: 17625241]
[44]
Soetan, K.O. Pharmacological and other beneficial effects of antinutritional factors in plants: a review. Afr. J. Biotechnol., 2008, 7, 4713-4721.
[45]
Ikewuchi, J.C.; Onyeike, E.N.; Uwakwe, A.A.; Ikewuchi, C.C. Effect of aqueous extract of the leaves of Acalypha wilkesiana ‘Godseffiana’ Muell Arg (Euphorbiaceae) on the hematology, plasma biochemistry and ocular indices of oxidative stress in alloxan induced diabetic rats. J. Ethnopharmacol., 2011, 137(3), 1415-1424.
[http://dx.doi.org/10.1016/j.jep.2011.08.015] [PMID: 21864665]
[46]
Ikewuchi, J.C.; Ikewuchi, C.C. Hypoglycemic, hypocholesterolemic and ocular-protective effects of an aqueous extract of the rhizomes of Sansevieria senegambica Baker (Agavaceae) on alloxan-induced diabetic Wistar rats. Am. J. Biochem. Mol. Biol, 2012, 2, 48-66.
[http://dx.doi.org/10.3923/ajbmb.2012.48.66]
[47]
Chigozie, I.J.; Chidinma, I.C. Positive moderation of the hematology, plasma biochemistry and ocular indices of oxidative stress in alloxan-induced diabetic rats, by an aqueous extract of the leaves of Sansevieria liberica Gerome and Labroy. Asian Pac. J. Trop. Med., 2013, 6(1), 27-36.
[http://dx.doi.org/10.1016/S1995-7645(12)60196-5] [PMID: 23317882]
[48]
Ikewuchi, J.C.; Ikewuchi, C.C. Hypoglycemic, hypocholesterolemic, anti-anemic and ocular-protective effects of an aqueous extract of the rhizomes of Sansevieria liberica Gérôme and Labroy (Agavaceae) on alloxan-induced diabetic Wistar rats. Asian J. Pharmacy Technol., 2011, 1, 137-148.
[49]
Ikewuchi, J.C.; Ikewuchi, C.C.; Ifeanacho, M.O.; Igboh, N.M.; Enuneku, E.C.; Osayande, O.I. Moderation of the plasma chemistry and hematological indices of normal and salt-loaded rats by an aqueous extract of the rhizomes of Sansevieria liberica: Implications for the management of hypertension and associated complications. Asian J. Pharmaceut. Res, 2013, 3, 134-140.
[50]
Dirsch, V.; Lacaille-Dubois, M-A.; Wagner, H. Search for the antirheumatic principle in the roots of Helleborus purpurascens. Planta Med., 1993, 59(Suppl.), A586.
[http://dx.doi.org/10.1055/s-2006-959786]
[51]
Moreno, P.R.; Falk, E.; Palacios, I.F.; Newell, J.B.; Fuster, V.; Fallon, J.T. Macrophage infiltration in acute coronary syndromes. Implications for plaque rupture. Circulation, 1994, 90(2), 775-778.
[http://dx.doi.org/10.1161/01.CIR.90.2.775] [PMID: 8044947]
[52]
Libby, P. Current concepts of the pathogenesis of the acute coronary syndromes. Circulation, 2001, 104(3), 365-372.
[http://dx.doi.org/10.1161/01.CIR.104.3.365] [PMID: 11457759]
[53]
Ayalogu, E.O.; Ikewuchi, C.C.; Onyeike, E.N.; Ikewuchi, J.C. Effect of aqueous extract of Sansevieria senegambica Baker on plasma biochemistry and hematological indices of salt-loaded rats. S. Afr. J. Sci., 2011, 107, 481.
[http://dx.doi.org/10.4102/sajs.v107i11/12.481]
[54]
Ikewuchi, J.C.; Ikewuchi, C.C. Moderation of haematological indices, plasma electrolytes and markers of hepato-renal function in sub-chronic salt-loaded rats by an aqueous leaf extract of Tridax procumbens Linn (Asteraceae). Pac. J. Sci. Technol., 2013, 14, 362-369.
[55]
Takeda, Y.; Suzuki, S.; Fukutomi, T.; Kondo, H.; Sugiura, M.; Suzumura, H.; Murasaki, G.; Okutani, H.; Itoh, M. Elevated white blood cell count as a risk factor of coronary artery disease: inconsistency between forms of the disease. Jpn. Heart J., 2003, 44(2), 201-211.
[http://dx.doi.org/10.1536/jhj.44.201] [PMID: 12718482]
[56]
Horne, B.D.; Anderson, J.L.; John, J.M.; Weaver, A.; Bair, T.L.; Jensen, K.R.; Renlund, D.G.; Muhlestein, J.B. Intermountain Heart Collaborative Study Group. Which white blood cell subtypes predict increased cardiovascular risk? J. Am. Coll. Cardiol., 2005, 45(10), 1638-1643.
[http://dx.doi.org/10.1016/j.jacc.2005.02.054] [PMID: 15893180]
[57]
Taniguchi, A.; Fukushima, M.; Seino, Y.; Sakai, M.; Yoshii, S.; Nagasaka, S.; Yamauchi, I.; Okumura, T.; Nin, K.; Tokuyama, K.; Yamadori, N.; Ogura, M.; Kuroe, A.; Nakai, Y. Platelet count is independently associated with insulin resistance in non-obese Japanese type 2 diabetic patients. Metabolism, 2003, 52(10), 1246-1249.
[http://dx.doi.org/10.1016/S0026-0495(03)00099-4] [PMID: 14564674]
[58]
Kaushansky, K. Determinants of platelet number and regulation of thrombopoiesis. Hematology (Am. Soc. Hematol. Educ. Program), 2009, 1, 147-152.
[http://dx.doi.org/10.1182/asheducation-2009.1.147] [PMID: 20008193]

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