Abstract
In this paper we report the development of chromatographic methods for the separation of 8 biologically active hydroxycholesterols (OHC’s) which include the single-hydroxyl addition species 7α-OHC, 7β-OHC, 25-OHC and 27- OHC, together with the double-hydroxyl addition species 7α, 25-OHC, 7β, 25-OHC, 7α, 27-OHC, and 7β, 27-OHC. Four complementary techniques were employed (gas chromatography, normal phase and reversed phase high performance liquid chromatography, and supercritical fluid chromatography), and for each of the techniques, an optimized method for the separation of all eight compounds in a mixture is presented.
Keywords: Oxycholesterol separation, oxysterol separation, GC (gas chromatography), HPLC (high performance liquid chromatography), SFC (supercritical fluid chromatography)
Current Topics in Medicinal Chemistry
Title:Chromatographic Separation of Bioactive Oxycholesterols by GC, HPLC and SFC
Volume: 12 Issue: 11
Author(s): Heather McAllister and Jiejun Wu
Affiliation:
Keywords: Oxycholesterol separation, oxysterol separation, GC (gas chromatography), HPLC (high performance liquid chromatography), SFC (supercritical fluid chromatography)
Abstract: In this paper we report the development of chromatographic methods for the separation of 8 biologically active hydroxycholesterols (OHC’s) which include the single-hydroxyl addition species 7α-OHC, 7β-OHC, 25-OHC and 27- OHC, together with the double-hydroxyl addition species 7α, 25-OHC, 7β, 25-OHC, 7α, 27-OHC, and 7β, 27-OHC. Four complementary techniques were employed (gas chromatography, normal phase and reversed phase high performance liquid chromatography, and supercritical fluid chromatography), and for each of the techniques, an optimized method for the separation of all eight compounds in a mixture is presented.
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Cite this article as:
McAllister Heather and Wu Jiejun, Chromatographic Separation of Bioactive Oxycholesterols by GC, HPLC and SFC, Current Topics in Medicinal Chemistry 2012; 12 (11) . https://dx.doi.org/10.2174/156802612800672880
DOI https://dx.doi.org/10.2174/156802612800672880 |
Print ISSN 1568-0266 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4294 |
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