Abstract
The binding of fisetin with human serum albumin (HSA) has been studied at different pH using UV-Vis, FTIR, CD and fluorescence spectroscopic techniques. The binding constants were found to increase with the rise in pH of the media. The negative ΔH° (kJ mol-1) and positive ΔS° (J mol-1 K-1) indicate that fisetin binds to HSA via electrostatic interactions with an initial hydrophobic association that result in a positive ΔS°. In presence of potassium chloride (KCl) the binding constants were found to be decrease. The α-helical content of HSA increased after binding with fisetin as analyzed from both CD and FTIR methods. The site marker displacement studies using fluorescence anisotropy suggest that fisetin binds to the hydrophobic pocket (Site 1, subdomain IIA) of HSA which is in good accordance with the molecular docking study. The change in accessible surface area (ASA) of residues of HSA was calculated to get a better insight into the binding.
Keywords: Binding, docking, fisetin, fluorescence and human serum albumin, antioxidant agents, ischemia, atherosclerosis, cardiovascular disease, human mast cells (HMC-1)
Protein & Peptide Letters
Title:Study of the Interaction Between Fisetin and Human Serum Albumin: A Biophysical Approach
Volume: 19 Issue: 6
Author(s): Atanu Singha Roy, Amit Kumar Dinda and Swagata Dasgupta
Affiliation:
Keywords: Binding, docking, fisetin, fluorescence and human serum albumin, antioxidant agents, ischemia, atherosclerosis, cardiovascular disease, human mast cells (HMC-1)
Abstract: The binding of fisetin with human serum albumin (HSA) has been studied at different pH using UV-Vis, FTIR, CD and fluorescence spectroscopic techniques. The binding constants were found to increase with the rise in pH of the media. The negative ΔH° (kJ mol-1) and positive ΔS° (J mol-1 K-1) indicate that fisetin binds to HSA via electrostatic interactions with an initial hydrophobic association that result in a positive ΔS°. In presence of potassium chloride (KCl) the binding constants were found to be decrease. The α-helical content of HSA increased after binding with fisetin as analyzed from both CD and FTIR methods. The site marker displacement studies using fluorescence anisotropy suggest that fisetin binds to the hydrophobic pocket (Site 1, subdomain IIA) of HSA which is in good accordance with the molecular docking study. The change in accessible surface area (ASA) of residues of HSA was calculated to get a better insight into the binding.
Export Options
About this article
Cite this article as:
Singha Roy Atanu, Kumar Dinda Amit and Dasgupta Swagata, Study of the Interaction Between Fisetin and Human Serum Albumin: A Biophysical Approach, Protein & Peptide Letters 2012; 19 (6) . https://dx.doi.org/10.2174/092986612800493995
DOI https://dx.doi.org/10.2174/092986612800493995 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Personalized and Regenerative Medicine for Liver Diseases
Current Stem Cell Research & Therapy Safflor Protected Rat Cerebral Ischemia-Reperfusion Injury Through Inhibiting the Expression of NF-kB and IL-1β
Neuroscience and Biomedical Engineering (Discontinued) Editorial (Thematic Issue: New Therapeutic Options for Anxiety and Mood Disorders)
CNS & Neurological Disorders - Drug Targets Innate Immunity in the Mucosal Immune System
Current Pharmaceutical Design Hck Inhibitors as Potential Therapeutic Agents in Cancer and HIV Infection
Current Medicinal Chemistry Efficacy of Duloxetine in Patients with Chronic Pain Conditions
Current Drug Therapy Targeting Lung Inflammation: Novel Therapies for the Treatment of COPD
Current Respiratory Medicine Reviews Meet Our Editorial Board Member
Recent Patents on Inflammation & Allergy Drug Discovery Characteristics Other than the Diagnostic Criteria Associated with Metabolic Syndrome: An Overview
Current Vascular Pharmacology Risk Factors for Myocardial Infarction in Women and Men: A Review of the Current Literature
Current Pharmaceutical Design Perivascular Inflammation and Hypertensive Cardiovascular Remodeling
Current Hypertension Reviews Patterns of Expression of Viral and Cytokine Gene Transcripts During Mouse Polyoma Virus Infection
Combinatorial Chemistry & High Throughput Screening Effects of HIV-1 Entry Inhibitors in Combination
Current Pharmaceutical Design Treatment with Paracetamol is not Associated with Increased Airway Sensitivity and Risk of Asthma in Rats
Current Drug Safety Analysis of Suicidal Behavior and Chronicity of Depressive Symptoms in the Presence of Hypovitaminosis D
Current Psychiatry Research and Reviews BMMSC-derived Exosomes Attenuate Cardiopulmonary Bypass-related Acute Lung Injury by Reducing Inflammatory Response and Oxidative Stress
Current Stem Cell Research & Therapy Recent Advances in Cancer Therapy: An Overview
Current Pharmaceutical Design A Glimpse of Matrix Metalloproteinases in Diabetic Nephropathy
Current Medicinal Chemistry Tuberculosis Infection Under Anti-TNF Alpha Treatment
Current Drug Safety Protease-Activated Receptor-2 Antagonists and Agonists
Current Medicinal Chemistry - Cardiovascular & Hematological Agents