Abstract
Various protein factors, including telomerase and WRN helicase, are involved in telomere maintenance. Resveratrol (Rsv), a polyphenol that extends the lifespan of diverse species is an activator of SIRT1, a NAD+ dependent deacetylating enzyme in mammalian cells. Here, we examined the changes in gene expressions and promoter activities of WRN helicase and telomerase after Rsv treatment. This treatment increased the amount of WRN transcript and protein product by activating its promoter and telomerase promoter activity and gene expression. However cell proliferation was not changed. This suggests that Rsv induces telomere maintenance factors like WRN helicase without affecting cell proliferation.
Keywords: BLM, Resveratrol, Telomerase, Sp1, TERT, WRN, Werner Syndrome, deacetylating enzyme, cell proliferation, eukaryotic chromosomes, fibroblasts, Reactive oxygen species (ROS), mitochondrial respiration, caloric restriction (CR), superoxide dismutases (SODs), Human cervical carcinoma (HeLa S3), Dulbecco's modified Eagle's (DME) medium, MTS assay, Luciferase, plasmids, DEAE-dextran method, Reverse Transcriptase Polymerase Chain Reaction (RTPCR), acrylamide gels, ethidium bromide, Real time PCR analysis, GAPDH, plasmid-transfected cells, anti-actin, anti-Sp1 antibodies, DNA-damage responses, DNA-repair synthesis, insulin signaling, epigenetic, sirtuin (SIRT1)-mediated deacetylation
Current Aging Science
Title: The Effect of Resveratrol on the Werner Syndrome RecQ Helicase Gene and Telomerase Activity
Volume: 4 Issue: 1
Author(s): Fumiaki Uchiumi, Takeshi Watanabe, Shin Hasegawa, Taisuke Hoshi, Yoshikazu Higami and Sei-ichi Tanuma
Affiliation:
Keywords: BLM, Resveratrol, Telomerase, Sp1, TERT, WRN, Werner Syndrome, deacetylating enzyme, cell proliferation, eukaryotic chromosomes, fibroblasts, Reactive oxygen species (ROS), mitochondrial respiration, caloric restriction (CR), superoxide dismutases (SODs), Human cervical carcinoma (HeLa S3), Dulbecco's modified Eagle's (DME) medium, MTS assay, Luciferase, plasmids, DEAE-dextran method, Reverse Transcriptase Polymerase Chain Reaction (RTPCR), acrylamide gels, ethidium bromide, Real time PCR analysis, GAPDH, plasmid-transfected cells, anti-actin, anti-Sp1 antibodies, DNA-damage responses, DNA-repair synthesis, insulin signaling, epigenetic, sirtuin (SIRT1)-mediated deacetylation
Abstract: Various protein factors, including telomerase and WRN helicase, are involved in telomere maintenance. Resveratrol (Rsv), a polyphenol that extends the lifespan of diverse species is an activator of SIRT1, a NAD+ dependent deacetylating enzyme in mammalian cells. Here, we examined the changes in gene expressions and promoter activities of WRN helicase and telomerase after Rsv treatment. This treatment increased the amount of WRN transcript and protein product by activating its promoter and telomerase promoter activity and gene expression. However cell proliferation was not changed. This suggests that Rsv induces telomere maintenance factors like WRN helicase without affecting cell proliferation.
Export Options
About this article
Cite this article as:
Uchiumi Fumiaki, Watanabe Takeshi, Hasegawa Shin, Hoshi Taisuke, Higami Yoshikazu and Tanuma Sei-ichi, The Effect of Resveratrol on the Werner Syndrome RecQ Helicase Gene and Telomerase Activity, Current Aging Science 2011; 4 (1) . https://dx.doi.org/10.2174/1874609811104010001
DOI https://dx.doi.org/10.2174/1874609811104010001 |
Print ISSN 1874-6098 |
Publisher Name Bentham Science Publisher |
Online ISSN 1874-6128 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Non-Invasive Tests in Animal Models and Humans: A New Paradigm for Assessing Efficacy of Biologics Including Prebiotics and Probiotics
Current Pharmaceutical Design Poly (Lactic-co-Glycolic Acid) & Tocopheryl Polyethylene Glycol Succinate Nanoparticles for the Treatment of Different Brain Cancers
Anti-Cancer Agents in Medicinal Chemistry Cardioprotection by Targeting the Pool of Resident and Extracardiac Progenitors
Current Drug Targets Biotransformations of Prenylated Hop Flavonoids for Drug Discovery and Production
Current Drug Metabolism Use of Transgenic Mice as Models for Prostate Cancer Chemoprevention
Current Molecular Medicine Melatonin Differentially Modulates NF-кB Expression in Breast and Liver Cancer Cells
Anti-Cancer Agents in Medicinal Chemistry Sympathetic Mechanisms of Hypoglycemic Counterregulation
Current Diabetes Reviews Clinical and Pharmacological Aspects of Inflammatory Demyelinating Diseases in Childhood: An Update
Current Neuropharmacology The Impact of Translational Research in Breast Cancer Care: Can we Improve the Therapeutic Scenario?
Anti-Cancer Agents in Medicinal Chemistry Neural Stem Cell Niches in Health and Diseases
Current Pharmaceutical Design Targeting Tumor Proteasome with Traditional Chinese Medicine
Current Drug Discovery Technologies Efficacy of Chemopreventive Agents in Mouse Mammary Gland Organ Culture (MMOC) Model: A Comprehensive Review
Current Medicinal Chemistry DNA Repair and Resistance to Topoisomerase I Inhibitors: Mechanisms, Biomarkers and Therapeutic Targets
Current Medicinal Chemistry Can Dietary Antioxidants Reduce the Incidence of Brain Tumors?
Current Drug Metabolism Medicinal Properties of Neem Leaves: A Review
Current Medicinal Chemistry - Anti-Cancer Agents Suppression of Oncoprotein Her-2 and DNA Damage after Treatment with Flavan-3- ol Vitis labrusca Extract
Anti-Cancer Agents in Medicinal Chemistry Biologics for the Treatment of Juvenile Idiopathic Arthritis
Current Medicinal Chemistry Intercellular Crosstalk Via Extracellular Vesicles in Tumor Milieu as Emerging Therapies for Cancer Progression
Current Pharmaceutical Design Medicinal Chemistry of P2X Receptors: Agonists and Orthosteric Antagonists
Current Medicinal Chemistry Physical Means of Stratum Corneum Barrier Manipulation to Enhance Transdermal Drug Delivery
Current Drug Delivery