Abstract
Effective and accurate analysis of free monosaccharides and monosaccharides bound to glycoconjugates, including glycoproteins and glycolipids have been achieved through the development of different analytical methods. Although all of these analytical methods appears to be applicable to the quantification of monosaccharides, quantification through coupling of liquid chromatography to mass spectrometry (LC-MS) is currently considered to be one the most effective analytical methods. This review focuses on the development of LC-MS methods for the analysis of monosaccharides, and evaluates the different analytical approaches including various ionization and detection techniques that have been used in order to analyze and quantify monosaccharides using mass spectrometry.
Keywords: monosaccharides, Glycans, High-performance liquid chromatography, LC-MS, glycoconjugates, glycoproteins, mass spectrometry, liquid chromatography, glycosylation, mammalian diseases
Current Proteomics
Title: Monosaccharide Compositional Analysis of Glycoproteins Using Liquid Chromatography-Mass Spectrometry
Volume: 8 Issue: 4
Author(s): Loubna A. Hammad and Yehia Mechref
Affiliation:
Keywords: monosaccharides, Glycans, High-performance liquid chromatography, LC-MS, glycoconjugates, glycoproteins, mass spectrometry, liquid chromatography, glycosylation, mammalian diseases
Abstract: Effective and accurate analysis of free monosaccharides and monosaccharides bound to glycoconjugates, including glycoproteins and glycolipids have been achieved through the development of different analytical methods. Although all of these analytical methods appears to be applicable to the quantification of monosaccharides, quantification through coupling of liquid chromatography to mass spectrometry (LC-MS) is currently considered to be one the most effective analytical methods. This review focuses on the development of LC-MS methods for the analysis of monosaccharides, and evaluates the different analytical approaches including various ionization and detection techniques that have been used in order to analyze and quantify monosaccharides using mass spectrometry.
Export Options
About this article
Cite this article as:
A. Hammad Loubna and Mechref Yehia, Monosaccharide Compositional Analysis of Glycoproteins Using Liquid Chromatography-Mass Spectrometry, Current Proteomics 2011; 8 (4) . https://dx.doi.org/10.2174/157016411798220835
DOI https://dx.doi.org/10.2174/157016411798220835 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
Call for Papers in Thematic Issues
Mass spectrometry data acquisition and analysis for proteomics
The Thematic Issue on "Mass spectrometry data acquisition and analysis for proteomics" aims to explore the latest advancements and challenges in the field of proteomics through the lens of mass spectrometry. Proteomics, the large-scale study of proteins and their functions, plays a crucial role in understanding various biological processes and ...read more
Peptides: State-of-Art and Commercialisation Hurdles
The Editors of the Current Proteomics (CP) journal are highly privileged to welcome scientists to submit their scientific research and review articles to be considered for publication in the upcoming thematic issue. The topics should cover various aspects of peptides in regard to their synthetic methodologies, formulation approaches, pharmacological challenges, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Light-at-Night-Induced Circadian Disruption, Cancer and Aging
Current Aging Science Molecular Bases of Chemoresistance in Cholangiocarcinoma
Current Drug Targets Multiple Mechanisms of Cytokine Action in Neurodegenerative and Psychiatric States: Neurochemical and Molecular Substrates
Current Pharmaceutical Design Notch Signaling Proteins: Legitimate Targets for Cancer Therapy
Current Protein & Peptide Science Targeting Phospholipase D-mediated Survival Signals in Cancer
Current Signal Transduction Therapy Phosphorylation Processes Controlling Aromatase Activity in Br east Cancer: An Update
Mini-Reviews in Medicinal Chemistry Digital Mammograms with Image Enhancement Techniques for Breast Cancer Detection: A Systematic Review
Current Medical Imaging Pharmacological Modulation of Caspase Activation
Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents Extranodal Marginal Zone B-Cell Lymphoma of Mucosa Associated Lymphoid Tissue Presenting as Multiple Pulmonary Lesions: Case Report and Review of the Literature
Current Respiratory Medicine Reviews Tumor Necrosis Factor Inhibitors in Pediatric Asthma
Recent Patents on Inflammation & Allergy Drug Discovery Retroviral Gene Therapy: Safety Issues and Possible Solutions
Current Gene Therapy Peroxisome Proliferator-Activated Receptors: Role of Isoform Gamma in the Antineoplastic Effect of Iodine in Mammary Cancer
Current Cancer Drug Targets Research Toward Potassium Channels on Tumor Progression
Current Topics in Medicinal Chemistry Patent Selections:
Recent Patents on Medical Imaging IL-2 Receptor Targeted Immunomodulatory Biologics: The Past, Present, and Future
Current Immunology Reviews (Discontinued) Immunosuppressive Treatments in Acute Myocardial Infarction and Stroke
Current Pharmaceutical Biotechnology Editorial: Primary Systemic Therapy for Breast Cancer
Reviews on Recent Clinical Trials Quantitative Structure-Activity Relationship Studies: Understanding the Mechanism of Tyrosine Kinase Inhibition
Current Enzyme Inhibition Opportunities and Challenges of Fluorescent Carbon Dots in Translational Optical Imaging
Current Pharmaceutical Design Investigating Physical, Psychological and Social Well-being of Older Persons in Jordan
Current Aging Science