Mechanism of Pain Relief by Low-Power Infrared Irradiation: ATP is an IR-Target Molecule in Nociceptive Neurons

Igor      L. Yachnev; Vera      B. Plakhova; Svetlana      A. Podzorova; Tatiana      N. Shelykh; Ilya      V. Rogachevsky; Boris      V. Krylov

Abstract

Effects of infrared (IR) radiation generated by a low-power Co2-laser on the membrane of cultured dissociated nociceptive neurons of newborn rat spinal ganglia were investigated using the whole-cell patch-clamp method. Lowpower IR radiation diminished the voltage sensitivity of activation gating machinery of slow sodium channels (Nav1.8). Ouabain known to block both transducer and pumping functions of Na+,K+-ATPase eliminated IR irradiation effects. The molecular mechanism of interaction of Co2-laser radiation with sensory membrane was proposed. The primary event of this interaction is the process of energy absorption by ATP molecules. The transfer of vibrational energy from Na+,K+- ATPase-bound and vibrationally excited ATP molecules to Na+,K+-ATPase activates this enzyme and converts it into a signal transducer. This effect leads to a decrease in the voltage sensitivity of Nav1.8 channels. The effect of IR-radiation was elucidated by the combined application of a very sensitive patch-clamp method and an optical facility with a controlled Co2-laser. As a result, the mechanism of interaction of non-thermal low-power IR radiation with the nociceptive neuron membrane is suggested.

Keywords: ATP molecules, infrared radiation, Na+,K+-ATPase, nociceptive neurons, patch-clamp method, 2-laser, G-proteins, glucose, embryo calf serum, gentamicin