Chemistry of SPOT Synthesis for the Preparation of Peptide Macroarrays on Cellulose Membranes

Dirk      F.H. Winkler

Abstract

The SPOT technique is one of the most frequently used methods for synthesis and screening of peptides on arrays. Materials such as polypropylene and glass are used for the preparation of peptide arrays, however the most commonly used material for SPOT membranes is cellulose. This paper focuses on materials and procedures used in the SPOT synthesis on cellulose membranes as a special type of solid phase peptide synthesis. In particular, different strategies for the modification of cellulose are described which make it more suitable for solid-phase peptide synthesis. This review also provides a short overview of the synthesis procedures including some important types of peptide modification.

Keywords: Cellulose membranes, linker, membrane modification, peptide array, solid phase peptide synthesis, SPOT synthesis, polypropylene, Glass