Abstract
Oxidative stress is caused by an imbalance between formation and destruction of reactive oxygen species. Analysis of the reaction products of reactive oxygen species in biomolecules is an indirect way of determining the existence of oxidative stress. In this context, the formation of carbonyl groups in proteins has been one of the most studied oxidative stress markers because of its stability and easy detection. Various proteomic tools offer great potential for the discovery of new proteins susceptible to oxidative stress, determination of quantitative changes in the profile of these modifications under different biological conditions, and characterization of the type of modification a particular protein has suffered. This paper reviews the different approaches used for the detection of protein carbonyls and the proteomic tools that can be used to identify them.
Keywords: Oxidative stress, protein oxidation, carbonyl groups, redox proteomics, proteomic tools, alpha amidation pathway, hydrazides, redox proteomase, mass spectrometry, protein carbonylation, Huntington disease
Current Protein & Peptide Science
Title: Proteomic Strategies for the Analysis of Carbonyl Groups on Proteins
Volume: 11 Issue: 8
Author(s): Veronica Irazusta, Armando Moreno-Cermeno, Elisa Cabiscol, Jordi Tamarit and Joaquim Ros
Affiliation:
Keywords: Oxidative stress, protein oxidation, carbonyl groups, redox proteomics, proteomic tools, alpha amidation pathway, hydrazides, redox proteomase, mass spectrometry, protein carbonylation, Huntington disease
Abstract: Oxidative stress is caused by an imbalance between formation and destruction of reactive oxygen species. Analysis of the reaction products of reactive oxygen species in biomolecules is an indirect way of determining the existence of oxidative stress. In this context, the formation of carbonyl groups in proteins has been one of the most studied oxidative stress markers because of its stability and easy detection. Various proteomic tools offer great potential for the discovery of new proteins susceptible to oxidative stress, determination of quantitative changes in the profile of these modifications under different biological conditions, and characterization of the type of modification a particular protein has suffered. This paper reviews the different approaches used for the detection of protein carbonyls and the proteomic tools that can be used to identify them.
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Cite this article as:
Irazusta Veronica, Moreno-Cermeno Armando, Cabiscol Elisa, Tamarit Jordi and Ros Joaquim, Proteomic Strategies for the Analysis of Carbonyl Groups on Proteins, Current Protein & Peptide Science 2010; 11 (8) . https://dx.doi.org/10.2174/138920310794557664
DOI https://dx.doi.org/10.2174/138920310794557664 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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