Abstract
The HSV1-tk gene has been explored as a reporter and/or suicide gene in molecular imaging of gene expression. Gene therapy with HSV1-tk and the use of this gene as a marker have been applied in patients with various forms of cancer. However, the conditions for clinical gene therapy protocols have yet to be optimized. A method to monitor the activity of HSV1-tk in vivo would be extremely useful to optimize clinical gene therapy protocols. Positron emission tomography (PET) with a suitable probe can offer information about both the extent of gene expression and its distribution, provided that an appropriate reporter gene is included in the therapeutic cassette. PET imaging provides higher resolution and sensitivity and allows noninvasive quantification of biological processes. Several radiolabeled pyrimidine (thymidine) and purine (acycloguanosine) derivatives have been developed as reporter probes for imaging of HSV1-TK enzyme activity with PET. In this review, information on radiolabeling and PET imaging of HSV1-tk gene expression with various nucleoside analogues is presented.
Keywords: 18F-Nucleoside, PET, Reporter gene, HSV1-tk, small molecules, gene expression, Gene therapy, Positron emission tomography (PET), ganciclovir (GCV), pyrimidine nucleoside analogues, purine (acycloguanosine) analogues, Purine Nucleoside Analogues, 18F-Pyrimidine Analogues, PET IMAGING, 18F-Purine Analogues, PURINE-BASED PROBES, CTLs, (SPECT), (18F-FET), 76Br-FBAU, 18F-FDG, 14C-FIAU, RG2 cells
Current Topics in Medicinal Chemistry
Title: Radiolabeled Nucleoside Analogues for PET Imaging of HSV1-tk Gene Expression
Volume: 10 Issue: 16
Author(s): Mian M. Alauddin and Juri G. Gelovani
Affiliation:
Keywords: 18F-Nucleoside, PET, Reporter gene, HSV1-tk, small molecules, gene expression, Gene therapy, Positron emission tomography (PET), ganciclovir (GCV), pyrimidine nucleoside analogues, purine (acycloguanosine) analogues, Purine Nucleoside Analogues, 18F-Pyrimidine Analogues, PET IMAGING, 18F-Purine Analogues, PURINE-BASED PROBES, CTLs, (SPECT), (18F-FET), 76Br-FBAU, 18F-FDG, 14C-FIAU, RG2 cells
Abstract: The HSV1-tk gene has been explored as a reporter and/or suicide gene in molecular imaging of gene expression. Gene therapy with HSV1-tk and the use of this gene as a marker have been applied in patients with various forms of cancer. However, the conditions for clinical gene therapy protocols have yet to be optimized. A method to monitor the activity of HSV1-tk in vivo would be extremely useful to optimize clinical gene therapy protocols. Positron emission tomography (PET) with a suitable probe can offer information about both the extent of gene expression and its distribution, provided that an appropriate reporter gene is included in the therapeutic cassette. PET imaging provides higher resolution and sensitivity and allows noninvasive quantification of biological processes. Several radiolabeled pyrimidine (thymidine) and purine (acycloguanosine) derivatives have been developed as reporter probes for imaging of HSV1-TK enzyme activity with PET. In this review, information on radiolabeling and PET imaging of HSV1-tk gene expression with various nucleoside analogues is presented.
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Cite this article as:
M. Alauddin Mian and G. Gelovani Juri, Radiolabeled Nucleoside Analogues for PET Imaging of HSV1-tk Gene Expression, Current Topics in Medicinal Chemistry 2010; 10 (16) . https://dx.doi.org/10.2174/156802610793176756
DOI https://dx.doi.org/10.2174/156802610793176756 |
Print ISSN 1568-0266 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4294 |
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