Abstract
The pressing demand to elucidate the biology of human embryonic stem (ES) cells and to realize their therapeutic potential has greatly promoted the progresses in the optimization of the culture systems used for this highly promising cell type. These progresses include the characterization of exogenous regulators of pluripotency and differentiation, the development of animal-free, defined, and scalable culture systems, and some pioneering efforts to establish good manufactory practice facilities to derive and expand clinical-grade human ES cells and their derivatives. All of these advancements appear to be also applicable to the derivation and culture of human induced pluripotent stem cells, an ES cell-like cell type derived from somatic cells via reprogramming. This review attempts to summarize these progresses and discuss some of the remaining challenges.
Keywords: Human embryonic stem cells, induced pluripotent stem cells, growth factors, tissue culture
Current Stem Cell Research & Therapy
Title: Progresses and Challenges in Optimization of Human Pluripotent Stem Cell Culture
Volume: 5 Issue: 3
Author(s): Ge Lin and Ren-He Xu
Affiliation:
Keywords: Human embryonic stem cells, induced pluripotent stem cells, growth factors, tissue culture
Abstract: The pressing demand to elucidate the biology of human embryonic stem (ES) cells and to realize their therapeutic potential has greatly promoted the progresses in the optimization of the culture systems used for this highly promising cell type. These progresses include the characterization of exogenous regulators of pluripotency and differentiation, the development of animal-free, defined, and scalable culture systems, and some pioneering efforts to establish good manufactory practice facilities to derive and expand clinical-grade human ES cells and their derivatives. All of these advancements appear to be also applicable to the derivation and culture of human induced pluripotent stem cells, an ES cell-like cell type derived from somatic cells via reprogramming. This review attempts to summarize these progresses and discuss some of the remaining challenges.
Export Options
About this article
Cite this article as:
Lin Ge and Xu Ren-He, Progresses and Challenges in Optimization of Human Pluripotent Stem Cell Culture, Current Stem Cell Research & Therapy 2010; 5 (3) . https://dx.doi.org/10.2174/157488810791824548
DOI https://dx.doi.org/10.2174/157488810791824548 |
Print ISSN 1574-888X |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-3946 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Insulin Resistance in Type 2 Diabetes - Role of the Adipokines
Current Molecular Medicine Amyloidogenicity and Aggregate Cytotoxicity of Human Glucagon-Like Peptide-1 (hGLP-1)
Protein & Peptide Letters Systemic Cytokines in Type 2 Diabetes Mellitus and Chronic Periodontitis
Current Diabetes Reviews Chymase as an Important Target for Preventing Complications of Metabolic Syndrome
Current Medicinal Chemistry Monitoring Calcific Aortic Valve Disease: The Role of Biomarkers
Current Medicinal Chemistry Interactions of Biologically Active Factors and Vascular Mediators During Hypertension in Pregnancy
Current Hypertension Reviews Jumping on the Train of Personalized Medicine: A Primer for Non-Geneticist Clinicians: Part 2. Fundamental Concepts in Genetic Epidemiology
Current Psychiatry Reviews Editorial (Thematic Issue: Metabolic Syndrome in Pregnancy)
Current Pharmaceutical Biotechnology Synthesis and Antioxidant Evaluation of a New Class of Thienopyrimidine-rhodanine Hybrids
Letters in Drug Design & Discovery Computational Advances in Chronic Diseases Diagnostics and Therapy - II
Current Drug Targets Hyaluronic Acid: Targeting Immune Modulatory Components of the Extracellular Matrix in Atherosclerosis
Current Medicinal Chemistry Editorial (Thematic Issue: Sex, Environment, Physical Activity, Diet, and Obesity in Cardiovascular Disease Risk)
Current Vascular Pharmacology Molecular Mechanisms of Renal Blood Flow Autoregulation
Current Vascular Pharmacology Inflammation and Mood Disorders: Proinflammatory Cytokines and the Pathogenesis of Depression
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Immunosuppressive Properties of Mesenchymal Stem Cells: Advances and Applications
Current Molecular Medicine Plasma-Free Amino Acid Profiling of Nasal Polyposis Patients
Combinatorial Chemistry & High Throughput Screening Serum Endocan, Neuron-Specific Enolase and Ischemia-Modified Albumin Levels in Newborns with Partial Blood Exchange Transfusion
Combinatorial Chemistry & High Throughput Screening ESVS Guidelines: Section B - Diagnosis and Investigation of Patients with Carotid Stenosis
Current Vascular Pharmacology The Negative Influence of Socio-Technical Developments on Eating Habits and Health
Current Nutrition & Food Science Human Amylin: From Pathology to Physiology and Pharmacology
Current Protein & Peptide Science