One-Step Purification of Functional Light-Harvesting 2 Complex from Rhodobacter sphaeroides

Zhiping      Zhao; Zongli      Hu; Yan      Liang; Tingzhang      Hu; Yun      Tu; Guoping      Chen

Abstract

Most common methods for isolation of the light-harvesting 2 (LH2) complex are time-consuming. In our present work, functional LH2 complex was one-step purified from Rhodobacter sphaeroides by immobilized metal-ion affinity chromatography. The purified LH2 complex exhibited high activity in light absorption and energy transfer. Our present work provides an efficient approach for preparation of functional LH2 complex.

Keywords: Light-harvesting 2 complexes, 10xHis-tag, light absorption, Rhodobacter sphaeroides

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Protein & Peptide Letters

Title: One-Step Purification of Functional Light-Harvesting 2 Complex from Rhodobacter sphaeroides

Volume: 17 Issue: 4

Author(s): Zhiping Zhao, Zongli Hu, Yan Liang, Tingzhang Hu, Yun Tu and Guoping Chen

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Keywords: Light-harvesting 2 complexes, 10xHis-tag, light absorption, Rhodobacter sphaeroides

Abstract: Most common methods for isolation of the light-harvesting 2 (LH2) complex are time-consuming. In our present work, functional LH2 complex was one-step purified from Rhodobacter sphaeroides by immobilized metal-ion affinity chromatography. The purified LH2 complex exhibited high activity in light absorption and energy transfer. Our present work provides an efficient approach for preparation of functional LH2 complex.

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Zhao Zhiping, Hu Zongli, Liang Yan, Hu Tingzhang, Tu Yun and Chen Guoping, One-Step Purification of Functional Light-Harvesting 2 Complex from Rhodobacter sphaeroides, Protein & Peptide Letters 2010; 17 (4) . https://dx.doi.org/10.2174/092986610790963663