Abstract
RNA interference (RNAi) is an attractive phenomenon for practical use that specifically inhibits gene expression and is carried out by small double-stranded RNAs (dsRNAs) including small interfering RNA (siRNA) or short hairpin RNA (shRNA). In addition, RNAi is of great interest for clinical use to cure refractory diseases related to the expression of a specific gene. To achieve gene silencing in the body, a sufficient amount of dsRNA must be delivered and internalized into target cells. However, dsRNAs have a large molecular weight and net negative charge, which limits their membrane-permeating ability. Moreover, dsRNAs are rapidly degraded by endonucleses in the body. Therefore, for the efficient delivery of dsRNAs, many approaches based on drug delivery systems have been carried out. In this review, we focus on recent reports about the application of functional peptides and proteins designed for the efficient delivery of dsRNAs.
Keywords: RNA interference (RNAi), gene delivery, double-stranded RNA (dsRNA), cell penetrating peptide (CPP), atelocollagen, double-stranded RNA-binding domain (dsRBD)
Current Topics in Medicinal Chemistry
Title: Enhanced Gene Delivery and/or Efficacy by Functional Peptide and Protein
Volume: 9 Issue: 12
Author(s): Tomoyuki Okuda, Yasuhisa Kawaguchi and Hirokazu Okamoto
Affiliation:
Keywords: RNA interference (RNAi), gene delivery, double-stranded RNA (dsRNA), cell penetrating peptide (CPP), atelocollagen, double-stranded RNA-binding domain (dsRBD)
Abstract: RNA interference (RNAi) is an attractive phenomenon for practical use that specifically inhibits gene expression and is carried out by small double-stranded RNAs (dsRNAs) including small interfering RNA (siRNA) or short hairpin RNA (shRNA). In addition, RNAi is of great interest for clinical use to cure refractory diseases related to the expression of a specific gene. To achieve gene silencing in the body, a sufficient amount of dsRNA must be delivered and internalized into target cells. However, dsRNAs have a large molecular weight and net negative charge, which limits their membrane-permeating ability. Moreover, dsRNAs are rapidly degraded by endonucleses in the body. Therefore, for the efficient delivery of dsRNAs, many approaches based on drug delivery systems have been carried out. In this review, we focus on recent reports about the application of functional peptides and proteins designed for the efficient delivery of dsRNAs.
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Cite this article as:
Okuda Tomoyuki, Kawaguchi Yasuhisa and Okamoto Hirokazu, Enhanced Gene Delivery and/or Efficacy by Functional Peptide and Protein, Current Topics in Medicinal Chemistry 2009; 9 (12) . https://dx.doi.org/10.2174/156802609789630857
DOI https://dx.doi.org/10.2174/156802609789630857 |
Print ISSN 1568-0266 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4294 |
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