Mitochondria-Targeted Green Fluorescent Protein for Quantitative Monitoring of Mitochondrial Morphology in Living Cells

Title: Mitochondria-Targeted Green Fluorescent Protein for Quantitative Monitoring of Mitochondrial Morphology in Living Cells

Volume: 6 Issue: 5

Author(s): Yasutomo Nomura, Toshifumi Takayama, Michihiko Sato, Eiji Takahashi, Zhonggang Feng and Takao Nakamura

Affiliation:

Keywords: Mitochondria, GFP, RH123, Image processing, Photobleaching

Abstract: In addition to Rhodamine 123, a progress in genetic engineering allowed the microscopic observation of mitochondria with GFP (mtGFP). Here we evaluated these labeling procedures for their ability to analyze mitochondrial morphology quantitatively. Expression of mtGFP was the optimal procedure to monitor mitochondrial morphology quantitatively, because of photo-stability and precise localization.

Cite this article as:

Nomura Yasutomo, Takayama Toshifumi, Sato Michihiko, Takahashi Eiji, Feng Zhonggang and Nakamura Takao, Mitochondria-Targeted Green Fluorescent Protein for Quantitative Monitoring of Mitochondrial Morphology in Living Cells, Letters in Drug Design & Discovery 2009; 6 (5) . https://dx.doi.org/10.2174/1570180810906050319