Abstract
miRNAs are a new class of non-coding small RNAs that exist in many species. They play important roles in many physiological and pathophysiological processes by inhibiting the expression of target RNAs. Recent advances in miRNAs are beginning to be predicted and identified using several technological approaches, such as miRNA cloning, hybridization with various probes, and PCR-based detection. In the past few years, miRNA microarray technology has become the reference technique for monitoring the miRNA expression. In this review, miRNAs will be introduced and the characteristics of normalization methods in miRNA research will be discussed. The technical operations of miRNA profiling with microarrays will also be described, with an emphasis on probe design and labelling. The applications of the miRNA microarray in both basic and applied research will be summarized.
Keywords: miRNAs, profiling, technology, microarray
Current Bioinformatics
Title: miRNA Microarray Technology in miRNA Profiling
Volume: 4 Issue: 2
Author(s): Shu-Ting Wang, Cai Li and Lei Liu
Affiliation:
Keywords: miRNAs, profiling, technology, microarray
Abstract: miRNAs are a new class of non-coding small RNAs that exist in many species. They play important roles in many physiological and pathophysiological processes by inhibiting the expression of target RNAs. Recent advances in miRNAs are beginning to be predicted and identified using several technological approaches, such as miRNA cloning, hybridization with various probes, and PCR-based detection. In the past few years, miRNA microarray technology has become the reference technique for monitoring the miRNA expression. In this review, miRNAs will be introduced and the characteristics of normalization methods in miRNA research will be discussed. The technical operations of miRNA profiling with microarrays will also be described, with an emphasis on probe design and labelling. The applications of the miRNA microarray in both basic and applied research will be summarized.
Export Options
About this article
Cite this article as:
Wang Shu-Ting, Li Cai and Liu Lei, miRNA Microarray Technology in miRNA Profiling, Current Bioinformatics 2009; 4 (2) . https://dx.doi.org/10.2174/157489309788184783
DOI https://dx.doi.org/10.2174/157489309788184783 |
Print ISSN 1574-8936 |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-392X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
From French Paradox to Cancer Treatment: Anti-cancer Activities and Mechanisms of Resveratrol
Anti-Cancer Agents in Medicinal Chemistry Advancing Metabolomics Research and Biomarker Application with Nanotechnology
Current Metabolomics Molecular Modeling, Docking, Dynamics and Simulation of Gefitinib and its Derivatives with EGFR in Non-small Cell Lung Cancer
Current Computer-Aided Drug Design FLT3 Inhibitors in the Management of Acute Myeloid Leukemia
Anti-Cancer Agents in Medicinal Chemistry Tetralone Scaffolds and Their Potential Therapeutic Applications
Letters in Drug Design & Discovery Omacetaxine as an Anticancer Therapeutic: What is Old is New Again
Current Pharmaceutical Design Regulation of Bcl-2 Family Proteins by Posttranslational Modifications
Current Molecular Medicine Immunotherapeutic and Immunoregulatory Drugs in Haematologic Malignancies
Current Topics in Medicinal Chemistry An Overview on Fibroblast Growth Factors: Structural, Functional and Therapeutic Implications
Current Proteomics Proteolytic Cleavage of Notch: “HIT and RUN”
Current Molecular Medicine Genetic Polymorphism and Tumor Immunotherapy
Current Pharmacogenomics Telomere Maintenance Mechanisms in Cancer: Clinical Implications
Current Pharmaceutical Design TAP-Independent MHC Class I Presentation
Current Immunology Reviews (Discontinued) Epigenetic Remodeling of Chromatin Architecture: Exploring Tumor Differentiation Therapies in Mesenchymal Stem Cells and Sarcomas
Current Stem Cell Research & Therapy Targeted Cancer Therapy; Nanotechnology Approaches for Overcoming Drug Resistance
Current Medicinal Chemistry Linc01559 Served as a Potential Oncogene and Promoted Resistance of Hepatocellular Carcinoma to Oxaliplatin by Directly Sponging miR-6783-3p
Anti-Cancer Agents in Medicinal Chemistry Exploring the Management of Statin Intolerant Patients: 2016 and Beyond
Current Vascular Pharmacology Variability of Biological Activities of Limonoids Derived from Plant Sources
Mini-Reviews in Organic Chemistry Synthesis and Preclinical Evaluation of Indole Triazole Conjugates as Microtubule Targeting Agents that are Effective against MCF-7 Breast Cancer Cell Lines
Anti-Cancer Agents in Medicinal Chemistry Perspectives Offered by Single-Domain Antibodies in Clinical Diagnostic of Pediatric Tumors
Current Medicinal Chemistry