Abstract
We have successfully expressed an active human brain serine racemase (hSR) with His-tag using moderate halophile. The purified His-hSR showed high elimination and racemization activities on L-serine: the elimination activity was 2.6-fold higher than racemization activity. Both enzyme activities showed an optimum reaction pH at around 9.0 and were stimulated 5- to 7-fold by such divalent cations as Mg++, Mn++ and Ca++.
Keywords: Human serine racemase, porin, groE, His-tag, halophile
Protein & Peptide Letters
Title: Purification and Characterization of Human Brain Serine Racemase Expressed in Moderately Halophilic Bacteria
Volume: 16 Issue: 2
Author(s): Chizuru Nagayoshi, Matsujiro Ishibashi and Masao Tokunaga
Affiliation:
Keywords: Human serine racemase, porin, groE, His-tag, halophile
Abstract: We have successfully expressed an active human brain serine racemase (hSR) with His-tag using moderate halophile. The purified His-hSR showed high elimination and racemization activities on L-serine: the elimination activity was 2.6-fold higher than racemization activity. Both enzyme activities showed an optimum reaction pH at around 9.0 and were stimulated 5- to 7-fold by such divalent cations as Mg++, Mn++ and Ca++.
Export Options
About this article
Cite this article as:
Nagayoshi Chizuru, Ishibashi Matsujiro and Tokunaga Masao, Purification and Characterization of Human Brain Serine Racemase Expressed in Moderately Halophilic Bacteria, Protein & Peptide Letters 2009; 16 (2) . https://dx.doi.org/10.2174/092986609787316261
DOI https://dx.doi.org/10.2174/092986609787316261 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Viral Inhibition of Tumour Necrosis Factor-α (TNFα) and TNF-Receptor Induced Apoptosis and Inflammation
Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents Conditional Reprogramming Inducing Clinical Cells Proliferation: New Research Tools in Tumor and Inflammatory-related Diseases
Current Pharmaceutical Design Drug Binding Domains of MRP1 (ABCC1) as Revealed by Photoaffinity Labeling
Current Medicinal Chemistry - Anti-Cancer Agents Host-Cell Surface Binding Targets in SARS-CoV-2 for Drug Design
Current Pharmaceutical Design Cyclooxygenase-2 Expression, Prostacyclin Production and Endothelial Protection of High-density Lipoprotein
Cardiovascular & Hematological Disorders-Drug Targets Current Perspectives on Anti-Aging Interventions
Letters in Drug Design & Discovery Recent Updates on Molecular Genetic Engineering Approaches and Applications of Human Therapeutic Proteins
Current Protein & Peptide Science Genetic Polymorphism in Allergic Rhinitis
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry NMR-based Phytochemical Profiling of Palmyra Palm Syrup Infused with Dry Ginger, Black Pepper, and Long Pepper
Current Nutraceuticals The Role of Inflammation in Epilepsy
Current Pediatric Reviews Anti-VEGF Therapy for Retinal Vein Occlusions
Current Drug Targets Mast Cells as Target in Cancer Therapy
Current Pharmaceutical Design Indoxyl Sulfate: A Candidate Target for the Prevention and Treatment of Cardiovascular Disease in Chronic Kidney Disease
Current Drug Targets Novel Risk Factors Related to Stable Angina
Current Pharmaceutical Design TRP Channels as Targets for Therapeutic Intervention in Obesity: Focus on TRPV1 and TRPM5
Current Topics in Medicinal Chemistry Sphingolipids in Genetic and Acquired Forms of Chronic Kidney Diseases
Current Medicinal Chemistry An Overview of Galenic Preparation Methods for Medicinal Cannabis
Current Bioactive Compounds Neuropathic Pain: Models and Mechanisms
Current Pharmaceutical Design Revised Roberts Cytoprotection and Adaptive Cytoprotection and Stable Gastric Pentadecapeptide BPC 157. Possible Significance and Implications for Novel Mediator
Current Pharmaceutical Design The SAPHO Syndrome and Genetics – discoveries in Need of Replication
Current Rheumatology Reviews