Generic placeholder image

Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Purification and Characterization of Human Brain Serine Racemase Expressed in Moderately Halophilic Bacteria

Author(s): Chizuru Nagayoshi, Matsujiro Ishibashi and Masao Tokunaga

Volume 16, Issue 2, 2009

Page: [201 - 206] Pages: 6

DOI: 10.2174/092986609787316261

Price: $65

Abstract

We have successfully expressed an active human brain serine racemase (hSR) with His-tag using moderate halophile. The purified His-hSR showed high elimination and racemization activities on L-serine: the elimination activity was 2.6-fold higher than racemization activity. Both enzyme activities showed an optimum reaction pH at around 9.0 and were stimulated 5- to 7-fold by such divalent cations as Mg++, Mn++ and Ca++.

Keywords: Human serine racemase, porin, groE, His-tag, halophile


Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy