Abstract
Conformational and structural changes of lentil seedlings amine oxidase (LSAO) were studied in the presence of trifluoroethanol (TFE) by spectroscopic and analytical techniques. At TFE concentrations up to 5%, the induction of a structural transition from β-sheet to α-helix and up to 10% TFE a structural transition from α-helix to β-sheet as well as inactivation of the enzyme are observed. At TFE concentrations between 10-35%, LSAO proves to be prone to aggregation and beyond 35% TFE leads to a non – native protein structure with a high α-helix content. The obtained results revealed that the aggregation of LSAO is strongly linked to the nature of secondary structures.
Keywords: Amine oxidase, TFE, Secondary structure, Circular dichroism, Fluorescence, Aggregation
Protein & Peptide Letters
Title: Structural Changes and Aggregation Process of Cu/Containing Amine Oxidase in the Presence of 2,2,2-Trifluoroethanol
Volume: 15 Issue: 5
Author(s): M. Amani, R. Yousefi, A. A. Moosavi-Movahedi, F. Pintus, A. Mura, G. Floris, B. I. Kurganov and A. A. Saboury
Affiliation:
Keywords: Amine oxidase, TFE, Secondary structure, Circular dichroism, Fluorescence, Aggregation
Abstract: Conformational and structural changes of lentil seedlings amine oxidase (LSAO) were studied in the presence of trifluoroethanol (TFE) by spectroscopic and analytical techniques. At TFE concentrations up to 5%, the induction of a structural transition from β-sheet to α-helix and up to 10% TFE a structural transition from α-helix to β-sheet as well as inactivation of the enzyme are observed. At TFE concentrations between 10-35%, LSAO proves to be prone to aggregation and beyond 35% TFE leads to a non – native protein structure with a high α-helix content. The obtained results revealed that the aggregation of LSAO is strongly linked to the nature of secondary structures.
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Amani M., Yousefi R., Moosavi-Movahedi A. A., Pintus F., Mura A., Floris G., Kurganov I. B. and Saboury A. A., Structural Changes and Aggregation Process of Cu/Containing Amine Oxidase in the Presence of 2,2,2-Trifluoroethanol, Protein & Peptide Letters 2008; 15 (5) . https://dx.doi.org/10.2174/092986608784567636
DOI https://dx.doi.org/10.2174/092986608784567636 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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