Abstract
In recent years, a strong emphasis has been given in deciphering the function of genes unraveled by the completion of several genome sequencing projects. In plants, functional genomics has been massively used in order to search for gene products of agronomic relevance. As far as root-pathogen interactions are concerned, several genes are recognized to provide tolerance/resistance against potential invaders. However, very few proteins have been identified by using current proteomic approaches. One of the major drawbacks for the successful analysis of root proteomes is the inherent characteristics of this tissue, which include low volume content and high concentration of interfering substances such as pigments and phenolic compounds. The proteome analysis of plant-pathogen interactions provides important information about the global proteins expressed in roots in response to biotic stresses. Moreover, several pathogenic proteins superimpose the plant proteome and can be identified and used as targets for the control of viruses, bacteria, fungi and nematode pathogens. The present review focuses on advances in different proteomic strategies dedicated to the challenging analysis of plant defense proteins expressed during bacteria-, fungi- and nematode-root interactions. Recent developments, limitations of the current techniques, and technological perspectives for root proteomics aiming at the identification of resistance- related proteins are discussed.
Keywords: Roots, proteome, 2DE, mass spectrometry
Current Protein & Peptide Science
Title: Rooteomics: The Challenge of Discovering Plant Defense-Related Proteins in Roots
Volume: 9 Issue: 2
Author(s): Angela Mehta, Beatriz S. Magalhaes, Djair S. L. Souza, Erico A. R. Vasconcelos, Luciano P. Silva, Maria Fatima Grossi-de-Sa, Octavio L. Franco, Paulo H. A. da Costa and Thales L. Rocha
Affiliation:
Keywords: Roots, proteome, 2DE, mass spectrometry
Abstract: In recent years, a strong emphasis has been given in deciphering the function of genes unraveled by the completion of several genome sequencing projects. In plants, functional genomics has been massively used in order to search for gene products of agronomic relevance. As far as root-pathogen interactions are concerned, several genes are recognized to provide tolerance/resistance against potential invaders. However, very few proteins have been identified by using current proteomic approaches. One of the major drawbacks for the successful analysis of root proteomes is the inherent characteristics of this tissue, which include low volume content and high concentration of interfering substances such as pigments and phenolic compounds. The proteome analysis of plant-pathogen interactions provides important information about the global proteins expressed in roots in response to biotic stresses. Moreover, several pathogenic proteins superimpose the plant proteome and can be identified and used as targets for the control of viruses, bacteria, fungi and nematode pathogens. The present review focuses on advances in different proteomic strategies dedicated to the challenging analysis of plant defense proteins expressed during bacteria-, fungi- and nematode-root interactions. Recent developments, limitations of the current techniques, and technological perspectives for root proteomics aiming at the identification of resistance- related proteins are discussed.
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Mehta Angela, Magalhaes S. Beatriz, Souza S. L. Djair, Vasconcelos A. R. Erico, Silva P. Luciano, Grossi-de-Sa Fatima Maria, Franco L. Octavio, da Costa H. A. Paulo and Rocha L. Thales, Rooteomics: The Challenge of Discovering Plant Defense-Related Proteins in Roots, Current Protein & Peptide Science 2008; 9 (2) . https://dx.doi.org/10.2174/138920308783955225
DOI https://dx.doi.org/10.2174/138920308783955225 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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