Abstract
One cDNA of a thrombin-like enzyme named acutinII was amplified by RT-PCR from the venom gland of the Agkistrodon acutus snake. AcutinII shows high similarity to acutin, the other thrombin-like enzyme from Agkistrodon acutus snake in nucleotide and amino acid sequences except it is shorter by 16 amino acid at the C-terminus. The catalytic residues and disulfide bridges of acutinII were deduced. AcutinII was expressed in E. coli .The expressed protein was detected by western blotting and the yield was up to 32 percent of the total proteins.
Keywords: CDNA CLONING, ACUTIN II, AGKISTRODON ACUTUS, Snake venom, Thrombin-like enzyme, RT-PCR, Expression, thrombosis
Protein & Peptide Letters
Title: Cdna Cloning And Expression Of Acutin Ii, A Native Mutant Of Thrombin-Like Enzyme From Agkistrodon Acutus
Volume: 8 Issue: 6
Author(s): Hua Pan, Jiewu Liu, Xiaoyan Du, Yuancong Zhou and Xiangfu Wu
Affiliation:
Keywords: CDNA CLONING, ACUTIN II, AGKISTRODON ACUTUS, Snake venom, Thrombin-like enzyme, RT-PCR, Expression, thrombosis
Abstract: One cDNA of a thrombin-like enzyme named acutinII was amplified by RT-PCR from the venom gland of the Agkistrodon acutus snake. AcutinII shows high similarity to acutin, the other thrombin-like enzyme from Agkistrodon acutus snake in nucleotide and amino acid sequences except it is shorter by 16 amino acid at the C-terminus. The catalytic residues and disulfide bridges of acutinII were deduced. AcutinII was expressed in E. coli .The expressed protein was detected by western blotting and the yield was up to 32 percent of the total proteins.
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Cite this article as:
Pan Hua, Liu Jiewu, Du Xiaoyan, Zhou Yuancong and Wu Xiangfu, Cdna Cloning And Expression Of Acutin Ii, A Native Mutant Of Thrombin-Like Enzyme From Agkistrodon Acutus, Protein & Peptide Letters 2001; 8 (6) . https://dx.doi.org/10.2174/0929866013409139
DOI https://dx.doi.org/10.2174/0929866013409139 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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