Purification and Characterization of a new Lectin from the Red Marine Alga Hypnea Musciformis

Title: Purification and Characterization of a new Lectin from the Red Marine Alga Hypnea Musciformis

Volume: 9 Issue: 2

Author(s): C. S. Nagano, F.B. M.B. Moreno, C. Bloch Jr, M. V. Prates, J. J. Calvete, S. Saker-Sampaio, W.R. L. Farias, T. D. Tavares, K. S. Nascimento, T. B. Grangeiro, B. S. Cavada and A. H. Sampaio

Affiliation:

Keywords: hypnea musciformis, red marine alga, lectin

Abstract: A lectin from the red marine alga Hypnea musciformis (HML) was purified by extraction with 20 mM PBS, precipitation with 70% saturated ammonium sulphate, ion-exchange DEAE-Cellulose chromatography and RP-HPLC. The 9.3 kDa polypeptide agglutinates erythrocytes from various sources and shows oligomerization tendencies under certain MALDI-TOF / MS conditions. Preliminary N-terminal sequencing and biological assays strongly suggest that the HML may belong to a new class of algae lectins.

Cite this article as:

Nagano S. C., Moreno M.B. F.B., Bloch Jr C., Prates V. M., Calvete J. J., Saker-Sampaio S., Farias L. W.R., Tavares D. T., Nascimento S. K., Grangeiro B. T., Cavada S. B. and Sampaio H. A., Purification and Characterization of a new Lectin from the Red Marine Alga Hypnea Musciformis, Protein & Peptide Letters 2002; 9 (2) . https://dx.doi.org/10.2174/0929866023408931