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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Crystallization And X-Ray Crystallographic Studies Of An Inhibitor From Rye (Secale Cereale) Active Against Acanthoscelides Obtectus And Zabrotes Subfasciatus Alpha-Amylases

Author(s): Jorge Iulek, Christiane Trevisan Slivinski and Marcio Silva

Volume 11, Issue 1, 2004

Page: [79 - 83] Pages: 5

DOI: 10.2174/0929866043478419

Price: $65

Abstract

Crystals of a new inhibitor present in rye seeds active against alpha-amylases from crop pests Acanthoscelides obtectus and Zabrotes subfasciatus have been obtained. A native dataset was collected at 2.21 Å resolution with 99.3 % completeness at CPr beamline at LNLS. The crystals belong to the trigonal system, space group P3121 with a = b = 78.21 Å, and c = 59.61 Å. The crystal calculated solvent content is compatible with one dimer per asymmetric unit.

Keywords: plant resistance against pests, crop improvement, cereal inhibitors, rye inhibitor, insect amylase inhibitor


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