Abstract
It has been recently shown that insulin retains its biological activity after receptor-directed internalization and it may affect the cell metabolism by interaction with cytosolic insulin-binding proteins (CIBPs). Using affinity chromatography combined with SDS-PAGE and MALDI-TOF mass-spectrometry we have identified 7 proteins from mouse liver cells that specifically bind to the insulin, including adenylate kinase 2 (25.6 kD), kinesin superfamily protein 20B (26.0 kD), hepatic arginase 1 (34.8 kD), fructosebisphosphate aldolase B (39.5 kD), 4-hydroxyphenylpyruvate dioxygenase (45.1 kD), betainehomocysteine methyl-transferase (45.0 kD) and KRIT1 (83.4 kD).
Keywords: insulin-binding protein, affinity chromatography, cytosol, liver cells
Protein & Peptide Letters
Title: Cytosolic Insulin-Binding Proteins Of Mouse Liver Cells
Volume: 11 Issue: 1
Author(s): Petr G. Lokhov, Sergei A. Moshkovskii, Olga M. Ipatova and Vladimir N. Prozorovskii
Affiliation:
Keywords: insulin-binding protein, affinity chromatography, cytosol, liver cells
Abstract: It has been recently shown that insulin retains its biological activity after receptor-directed internalization and it may affect the cell metabolism by interaction with cytosolic insulin-binding proteins (CIBPs). Using affinity chromatography combined with SDS-PAGE and MALDI-TOF mass-spectrometry we have identified 7 proteins from mouse liver cells that specifically bind to the insulin, including adenylate kinase 2 (25.6 kD), kinesin superfamily protein 20B (26.0 kD), hepatic arginase 1 (34.8 kD), fructosebisphosphate aldolase B (39.5 kD), 4-hydroxyphenylpyruvate dioxygenase (45.1 kD), betainehomocysteine methyl-transferase (45.0 kD) and KRIT1 (83.4 kD).
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Lokhov G. Petr, Moshkovskii A. Sergei, Ipatova M. Olga and Prozorovskii N. Vladimir, Cytosolic Insulin-Binding Proteins Of Mouse Liver Cells, Protein & Peptide Letters 2004; 11 (1) . https://dx.doi.org/10.2174/0929866043478356
DOI https://dx.doi.org/10.2174/0929866043478356 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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