Generic placeholder image

Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Stabilization of Cold-Adapted Protease MCP-01 Promoted by Trehalose: Prevention of the Autolysis

Author(s): Jun Pan, Xiu-Lan Chen, Cai-Yun Shun, Hai-Lun He and Yu-Zhong Zhang

Volume 12, Issue 4, 2005

Page: [375 - 378] Pages: 4

DOI: 10.2174/0929866053765626

Price: $65

Abstract

Protease MCP-01 is similar to other cold-adapted enzymes in that it is a cold-adapted serine protease having high specific activity and low thermostability at low and moderate temperature. Its thermolability and self-autolysis has resulted in difficulties in its purification, preservation and research on its structure and function. The disaccharide trehalose is known to effectively stabilize proteins. Its prevention effect on the autolysis of cold-adapted protease MCP-01 was monitored by capillary electrophoresis. In the absence of trehalose, protease MCP-01 autolyzed rapidly at 35°C. However, when trehalose was added, autolysis was remarkably prevented and the loss of activity reduced. MCP-01 may be a useful model for basic research on the interaction of protein and trehalose.

Keywords: autolysis, cold-adapted protease, trehalose


Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy