Intein-Mediated, In Vitro and In Vivo Protein Modifications with Small Molecules

Lay   P.   Tan; Shao   Q.   Yao

Author(s): Lay P. Tan and Shao Q. Yao

Volume 12, Issue 8, 2005

Page: [769 - 775] Pages: 7

DOI: 10.2174/0929866054864193

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Abstract

We review intein-mediated approaches for the site-specific modifications of proteins and highlight their applications in (1) the site-specific in vitro and in vivo biotinylation of proteins for protein arrays and (2) the site-specific in vivo labeling of proteins in living cells.

Keywords: intein, biotinylation, native chemical ligation, protein arrays, bioimaging, site-specific

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Protein & Peptide Letters

Title: Intein-Mediated, In Vitro and In Vivo Protein Modifications with Small Molecules

Volume: 12 Issue: 8

Author(s): Lay P. Tan and Shao Q. Yao

Affiliation:

Keywords: intein, biotinylation, native chemical ligation, protein arrays, bioimaging, site-specific

Abstract: We review intein-mediated approaches for the site-specific modifications of proteins and highlight their applications in (1) the site-specific in vitro and in vivo biotinylation of proteins for protein arrays and (2) the site-specific in vivo labeling of proteins in living cells.

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Tan P. Lay and Yao Q. Shao, Intein-Mediated, In Vitro and In Vivo Protein Modifications with Small Molecules, Protein & Peptide Letters 2005; 12 (8) . https://dx.doi.org/10.2174/0929866054864193