Abstract
Tobacco plants were transformed with gene constructs encoding prepro-ConBr (Canavalia brasiliensis lectin). Transgenic plants confirmed by PCR expressed the recombinant protein as revealed by Western blot. However, the apparent molecular mass of the recombinant polypeptide (ca. 34 kDa) was higher than the native lectin (about 30 kDa), showing that further proteolytic processing of pro-ConBr was not detected.
Keywords: Canavalia brasiliensis, lectin, recombinant, processing, heterelogous expression, precursor
Protein & Peptide Letters
Title: Expression of the Canavalia brasiliensis Lectin (ConBr) in Tobacco Plants
Volume: 13 Issue: 10
Author(s): Cristina Paiva da Silveira Carvalho, Tuana Oliveira Correia, Sandra Mara Serafim Ribeiro, Ingrid Cavalcante Silva, Jose Tadeu Abreu de Oliveira and Thalles Barbosa Grangeiro
Affiliation:
Keywords: Canavalia brasiliensis, lectin, recombinant, processing, heterelogous expression, precursor
Abstract: Tobacco plants were transformed with gene constructs encoding prepro-ConBr (Canavalia brasiliensis lectin). Transgenic plants confirmed by PCR expressed the recombinant protein as revealed by Western blot. However, the apparent molecular mass of the recombinant polypeptide (ca. 34 kDa) was higher than the native lectin (about 30 kDa), showing that further proteolytic processing of pro-ConBr was not detected.
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Cite this article as:
Paiva da Silveira Carvalho Cristina, Oliveira Correia Tuana, Mara Serafim Ribeiro Sandra, Cavalcante Silva Ingrid, Tadeu Abreu de Oliveira Jose and Barbosa Grangeiro Thalles, Expression of the Canavalia brasiliensis Lectin (ConBr) in Tobacco Plants, Protein & Peptide Letters 2006; 13 (10) . https://dx.doi.org/10.2174/092986606778777434
DOI https://dx.doi.org/10.2174/092986606778777434 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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