Methodological Improvements on Extraction of Nuclear Proteins and Its Preliminary Analysis During the Maize (Zea mays L.) Endosperm Development

Title: Methodological Improvements on Extraction of Nuclear Proteins and Its Preliminary Analysis During the Maize (Zea mays L.) Endosperm Development

Volume: 13 Issue: 10

Author(s): B. S. Ferreira, A. T. Branco, M. A. de Oliveira, M. G. Pereira and G. A. de Souza Filho

Affiliation:

Keywords: Nuclear proteins, endosperm development, and maize

Abstract: A procedure to obtain endosperm protein extracts was standardized. After confirming the enrichment with nuclear proteins by immunodetection, the protein profiles of extracts from different seed development stages were compared by SDS-PAGE that showed the existence of several differentially expressed proteins.

Cite this article as:

Ferreira S. B., Branco T. A., de Oliveira A. M., Pereira G. M. and de Souza Filho A. G., Methodological Improvements on Extraction of Nuclear Proteins and Its Preliminary Analysis During the Maize (Zea mays L.) Endosperm Development, Protein & Peptide Letters 2006; 13 (10) . https://dx.doi.org/10.2174/092986606778777470