Generic placeholder image

Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Two-Dimensional Gel Electrophoresis Based Technologies for Potential Biomarkers Identification in Amniotic Fluid: A Simple Model

Author(s): P.-A. Queloz, D. Crettaz, L. Thadikkaran, V. Sapin and J.-D. Tissot

Volume 13, Issue 10, 2006

Page: [959 - 963] Pages: 5

DOI: 10.2174/092986606778777506

Price: $65

Abstract

To assess the efficiency of two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and of twodimensional electrophoresis and ammoniacal silver staining (2D-E), different amounts of soybean trypsin inhibitor and horse myoglobin were added to amniotic fluid. In this model, a minimum of 5 to 10 ng of “artificial” biomarkers was detected.

Keywords: Amniotic fluid, Biomarker, CyDye, Proteomics, Two-dimensional electrophoresis


Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy