Abstract
A lectin from the seeds of Amaranthus viridis Linn has been purified by affinity chromatography on asialofetuin- linked amino activated silica. Amaranthus viridis lectin (AVL) has a native molecular mass of 67 kDa. It is a homodimer composed of two 36.6 kDa subunits. The lectin gave a single band in non-denaturing PAGE at pH 4.5 and pH 8.3 and a single peak on HPLC size exclusion and cation exchange columns. The purified lectin was specific for both Tantigen and N-acetyl-D-lactosamine, markers for various carcinomas, in addition to N-acetyl-D-galactosamine, asialofetuin and fetuin. This lectin reacted strongly with red blood cells (RBCs) from human ABO blood groups and rat. It also reacted with rabbit, sheep, goat and guinea pig RBCs. The lectin is a glycoprotein having no metal ion requirement for its activity. Denaturing agents such as urea, thiourea and guanidine-HCl had no effect on its activity when treated for 15 minutes. AVL showed significant antiproliferative activity towards HB98 and P388D1 murine cancer cell lines. It also exerted antifungal activity against phytopathogenic fungi Botrytis cincerea and Fusarium oxysporum but not against Rhizoctonia solani, Trichoderma reesei, Alternaria solani and Fusarium graminearum.
Keywords: Affinity purification, Amaranthus viridis, amaranthaceae, asialofetuin, N-acetyl-D-lactosamine, lectin, T-antigen
Protein & Peptide Letters
Title: A Novel Antiproliferative and Antifungal Lectin from Amaranthus viridis Linn Seeds
Volume: 13 Issue: 9
Author(s): Navjot Kaur, Vikram Dhuna, Sukhdev Singh Kamboj, Javed N Agrewala and Jatinder Singh
Affiliation:
Keywords: Affinity purification, Amaranthus viridis, amaranthaceae, asialofetuin, N-acetyl-D-lactosamine, lectin, T-antigen
Abstract: A lectin from the seeds of Amaranthus viridis Linn has been purified by affinity chromatography on asialofetuin- linked amino activated silica. Amaranthus viridis lectin (AVL) has a native molecular mass of 67 kDa. It is a homodimer composed of two 36.6 kDa subunits. The lectin gave a single band in non-denaturing PAGE at pH 4.5 and pH 8.3 and a single peak on HPLC size exclusion and cation exchange columns. The purified lectin was specific for both Tantigen and N-acetyl-D-lactosamine, markers for various carcinomas, in addition to N-acetyl-D-galactosamine, asialofetuin and fetuin. This lectin reacted strongly with red blood cells (RBCs) from human ABO blood groups and rat. It also reacted with rabbit, sheep, goat and guinea pig RBCs. The lectin is a glycoprotein having no metal ion requirement for its activity. Denaturing agents such as urea, thiourea and guanidine-HCl had no effect on its activity when treated for 15 minutes. AVL showed significant antiproliferative activity towards HB98 and P388D1 murine cancer cell lines. It also exerted antifungal activity against phytopathogenic fungi Botrytis cincerea and Fusarium oxysporum but not against Rhizoctonia solani, Trichoderma reesei, Alternaria solani and Fusarium graminearum.
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Kaur Navjot, Dhuna Vikram, Kamboj Sukhdev Singh, Agrewala N Javed and Singh Jatinder, A Novel Antiproliferative and Antifungal Lectin from Amaranthus viridis Linn Seeds, Protein & Peptide Letters 2006; 13 (9) . https://dx.doi.org/10.2174/092986606778256153
DOI https://dx.doi.org/10.2174/092986606778256153 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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