Abstract
A simple and sensitive isocratic reverse-phase high performance liquid chromatography method coupled with charged aerosol detection (HPLC-CAD) was developed and validated for the determination of gabapentin concentrations in a pharmaceutical formulation and in biological samples (rat serum and urine). Biological samples were simply treated with acetonitrile and directly injected into the HPLC without any further extraction or derivatization procedures. The separation was achieved on a GraceSmart RP-C18 packed column (250 × 4.6 mm, 5 μm) using a methanol-water (55:45, v/v) mobile phase at a flow rate of 1.1 mL/min at 30 °C. The total run time was less than 8 min. The standard calibration curves were linear (r2 > 0.998) over the range of 2-300 μg/mL for the pharmaceutical formulation, 25-800 μg/mL for rat serum and 50-1600 μg/mL for rat urine. The limits of detection (signal/noise = 3) were 0.5 μg/mL, 10 μg/mL and 15 μg/mL for pharmaceutical formulation, serum and urine, respectively. The intra-day and inter-day precisions in the pharmaceutical formulation analysis ranged between 2.7-3.9% and 3.1-4.4%, respectively. In the rat serum analysis, the intra-day and inter-day precision ranges were 3.5-4.7% and 3.0-9.0%, respectively, while the rat urine analysis intra-day and inter-day precision ranges were 1.9-7.6% and 4.2-9.9%, respectively. The developed HPLC-CAD method successfully determined gabapentin concentrations in commercial gabapentin tablets, as well as in rat serum and urine samples after drug administration.
Keywords: Charged aerosol detector, Commercial drug, Gabapentin, HPLC, Non-derivatization, Serum, Urine, HPLC-CAD, Anti-epileptic drug, Mass spectrometry, Flame ionization detection, Gas chromatography, Capillary electrophoresis
Current Analytical Chemistry
Title: Non-Derivatization Method for the Determination of Gabapentin in Pharmaceutical Formulations, Rat Serum and Rat Urine using High Performance Liquid Chromatography Coupled with Charged Aerosol Detection
Volume: 8 Issue: 1
Author(s): Shaodong Jia, Hee-Seung Lee, Myung-Joo Choi, Sang Hyun Sung, Sang B. Han, Jeong H. Park, Soon-Sun Hong, Sung W. Kwon and Jeongmi Lee
Affiliation:
Keywords: Charged aerosol detector, Commercial drug, Gabapentin, HPLC, Non-derivatization, Serum, Urine, HPLC-CAD, Anti-epileptic drug, Mass spectrometry, Flame ionization detection, Gas chromatography, Capillary electrophoresis
Abstract: A simple and sensitive isocratic reverse-phase high performance liquid chromatography method coupled with charged aerosol detection (HPLC-CAD) was developed and validated for the determination of gabapentin concentrations in a pharmaceutical formulation and in biological samples (rat serum and urine). Biological samples were simply treated with acetonitrile and directly injected into the HPLC without any further extraction or derivatization procedures. The separation was achieved on a GraceSmart RP-C18 packed column (250 × 4.6 mm, 5 μm) using a methanol-water (55:45, v/v) mobile phase at a flow rate of 1.1 mL/min at 30 °C. The total run time was less than 8 min. The standard calibration curves were linear (r2 > 0.998) over the range of 2-300 μg/mL for the pharmaceutical formulation, 25-800 μg/mL for rat serum and 50-1600 μg/mL for rat urine. The limits of detection (signal/noise = 3) were 0.5 μg/mL, 10 μg/mL and 15 μg/mL for pharmaceutical formulation, serum and urine, respectively. The intra-day and inter-day precisions in the pharmaceutical formulation analysis ranged between 2.7-3.9% and 3.1-4.4%, respectively. In the rat serum analysis, the intra-day and inter-day precision ranges were 3.5-4.7% and 3.0-9.0%, respectively, while the rat urine analysis intra-day and inter-day precision ranges were 1.9-7.6% and 4.2-9.9%, respectively. The developed HPLC-CAD method successfully determined gabapentin concentrations in commercial gabapentin tablets, as well as in rat serum and urine samples after drug administration.
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Cite this article as:
Jia Shaodong, Lee Hee-Seung, Choi Myung-Joo, Hyun Sung Sang, B. Han Sang, H. Park Jeong, Hong Soon-Sun, W. Kwon Sung and Lee Jeongmi, Non-Derivatization Method for the Determination of Gabapentin in Pharmaceutical Formulations, Rat Serum and Rat Urine using High Performance Liquid Chromatography Coupled with Charged Aerosol Detection, Current Analytical Chemistry 2012; 8 (1) . https://dx.doi.org/10.2174/157341112798472161
DOI https://dx.doi.org/10.2174/157341112798472161 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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