Abstract
Selected from random pools of DNA or RNA molecules through systematic evolution of ligands by exponential enrichment (SELEX), aptamers can bind to target molecules with high affinity and specificity, which makes them ideal recognition elements in the development of biosensors. To date, aptamer-based biosensors have used a wide variety of detection techniques, which are briefly summarized in this article. The focus of this review is on the development of aptamer-based fluorescent biosensors, with emphasis on their design as well as properties such as sensitivity and specificity. These biosensors can be broadly divided into two categories: those using fluorescently-labeled aptamers and others that employ label-free aptamers. Within each category, they can be further divided into “signalon” and “signal-off” sensors. A number of these aptamer-based fluorescent biosensors have shown promising results in biological samples such as urine and serum, suggesting their potential applications in biomedical research and disease diagnostics.
Keywords: Aptamers, biosensor, fluorescence, nanosensor, fluorescence resonance energy transfer (FRET)
Current Medicinal Chemistry
Title: Aptamer-Based Fluorescent Biosensors
Volume: 18 Issue: 27
Author(s): R. E. Wang, Y. Zhang, J. Cai, W. Cai and T. Gao
Affiliation:
Keywords: Aptamers, biosensor, fluorescence, nanosensor, fluorescence resonance energy transfer (FRET)
Abstract: Selected from random pools of DNA or RNA molecules through systematic evolution of ligands by exponential enrichment (SELEX), aptamers can bind to target molecules with high affinity and specificity, which makes them ideal recognition elements in the development of biosensors. To date, aptamer-based biosensors have used a wide variety of detection techniques, which are briefly summarized in this article. The focus of this review is on the development of aptamer-based fluorescent biosensors, with emphasis on their design as well as properties such as sensitivity and specificity. These biosensors can be broadly divided into two categories: those using fluorescently-labeled aptamers and others that employ label-free aptamers. Within each category, they can be further divided into “signalon” and “signal-off” sensors. A number of these aptamer-based fluorescent biosensors have shown promising results in biological samples such as urine and serum, suggesting their potential applications in biomedical research and disease diagnostics.
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Cite this article as:
E. Wang R., Zhang Y., Cai J., Cai W. and Gao T., Aptamer-Based Fluorescent Biosensors, Current Medicinal Chemistry 2011; 18 (27) . https://dx.doi.org/10.2174/092986711797189637
DOI https://dx.doi.org/10.2174/092986711797189637 |
Print ISSN 0929-8673 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-533X |
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