Abstract
Food allergy has become a serious human health concern. With more than 160 kinds of food allergens have been identified to date, allergen detection has become challenging. Most current allergen detection methods are capable of detecting only a few allergens at a time. The objective of this work was to develop a sensitive method for the detection of fish parvalbumin in food matrices employing a 3D aldehyde protein chip, which has the capacity to detect several allergens simultaneously. Upon testing, the protein chip was found to be highly specific in allergen detection but was found to cross-react to some extent with the allergens from some closely related fish species. The allergen recoveries ranged from 72.1% to 95.2%. The inter- and intra- assay coefficients of variation were < 18% and < 12% respectively. The LOD (limit of detection) and LOQ (limit of quantitation) of the protein chip of 0.048 and 0.087 mg parvalbumin/kg food, respectively, was sensitive enough given the threshold limit for the fish allergen. The newly developed assay seems reliable enough in detecting fish parvalbumin from various foods, food products and thus could prove useful in detecting and minimizing the instances of fish allergy.
Keywords: Allergen, Protein chip, Fish, Parvalbumin, Detection, Food allergy, 3D aldehyde protein chip, Limit of quantitation, Limit of detection, Threshold limit
Current Analytical Chemistry
Title: Development of an Optimized Protein Chip for the Detection of Fish Parvalbumin Allergen
Volume: 7 Issue: 4
Author(s): Zhenxing Li, Yiqun Zhang, Ravindra Pawar, Guoying Wang and Hong Lin
Affiliation:
Keywords: Allergen, Protein chip, Fish, Parvalbumin, Detection, Food allergy, 3D aldehyde protein chip, Limit of quantitation, Limit of detection, Threshold limit
Abstract: Food allergy has become a serious human health concern. With more than 160 kinds of food allergens have been identified to date, allergen detection has become challenging. Most current allergen detection methods are capable of detecting only a few allergens at a time. The objective of this work was to develop a sensitive method for the detection of fish parvalbumin in food matrices employing a 3D aldehyde protein chip, which has the capacity to detect several allergens simultaneously. Upon testing, the protein chip was found to be highly specific in allergen detection but was found to cross-react to some extent with the allergens from some closely related fish species. The allergen recoveries ranged from 72.1% to 95.2%. The inter- and intra- assay coefficients of variation were < 18% and < 12% respectively. The LOD (limit of detection) and LOQ (limit of quantitation) of the protein chip of 0.048 and 0.087 mg parvalbumin/kg food, respectively, was sensitive enough given the threshold limit for the fish allergen. The newly developed assay seems reliable enough in detecting fish parvalbumin from various foods, food products and thus could prove useful in detecting and minimizing the instances of fish allergy.
Export Options
About this article
Cite this article as:
Li Zhenxing, Zhang Yiqun, Pawar Ravindra, Wang Guoying and Lin Hong, Development of an Optimized Protein Chip for the Detection of Fish Parvalbumin Allergen, Current Analytical Chemistry 2011; 7 (4) . https://dx.doi.org/10.2174/157341111797183100
DOI https://dx.doi.org/10.2174/157341111797183100 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Advances in Microfluidic and Sensing Technologies of Biofluids.
Detection of biological fluids is an important means to assess human health and diagnose diseases. Currently, research on blood is relatively mature and widely applied in the medical field. With the development and advancement of microfluidic technology and novel detection methods, attention has turned to human physiological fluids such as ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Recent Patents on Antimicrobial Peptides
Recent Patents on DNA & Gene Sequences Plants Indicated by Brazilian Indians for Disturbances of the Central Nervous System: A Bibliographical Survey
Central Nervous System Agents in Medicinal Chemistry Immunomodulatory Activity of Punarnavine Alkaloid from <i>Boerhaavia diffusa</i>
Current Bioactive Compounds Ovarian Stimulation: Today and Tomorrow
Current Pharmaceutical Biotechnology Protective Effects of Low Dose Vorinostat on Cisplatin-Induced Nephrotoxicity in Rats
Current Molecular Pharmacology Quantitative Structure Activity Relationship Studies of Piperazinyl Phenylalanine Derivatives as VLA-4/VCAM-1 Inhibitors
Medicinal Chemistry The Role of IL-27 and its Receptor in the Pathogenesis of HIV/AIDS and Anti-viral Immune Response
Current HIV Research Maximizing Biomass and Lipid Production in Heterotrophic Culture of Chlorella vulgaris: Techno-Economic Assessment
Recent Patents on Food, Nutrition & Agriculture Novel PET Tracers in the Management of Cardiac Sarcoidosis
Current Radiopharmaceuticals Autoantibody-Induced Formation of Immune Complexes in Normal Human Serum
Current Pharmaceutical Design Editorial (Thematic Issue: Novel Therapeutic Strategies for Cardiovascular Disease Treatment: From Molecular Level to Nanotechnology)
Current Pharmaceutical Design The Role of Corticotropin-Releasing Hormone in the Pathophysiology of Depression: Therapeutic Implications
Current Topics in Medicinal Chemistry Protective Effects of Mesenchymal Stem Cells Against Central Nervous System Injury in Heat Stroke
Current Stem Cell Research & Therapy Targeted Radionuclide Therapy of Painful Bone Metastases: Past Developments, Current Status, Recent Advances and Future Directions
Current Medicinal Chemistry The Role of Transforming Growth Factor-β1 Gene Polymorphism and Its Serum Levels in Hashimoto’s Thyroiditis
Current Pharmaceutical Biotechnology Age Related Osteoarthritis: Regenerative Therapy, Synthetic Drugs, and Naturopathy to Combat Abnormal Signal Transduction
Current Signal Transduction Therapy Antithrombotic Treatment in the Prevention of Ischemic Stroke
Current Drug Targets FKBP Ligands as Novel Therapeutics for Neurological Disorders
Mini-Reviews in Medicinal Chemistry Testicular Cell Junction: A Novel Target for Male Contraception
Current Medicinal Chemistry Editorial:
Drug Metabolism Letters