Abstract
Interaction between prulifloxacin (PUFX) and pepsin was studied under a simulated physiological condition using fluorescence spectrophotometric techniques when the Eu(III) exists. The fluorescence emission intensity of pepsin was strongly quenched by the addition of prulifloxacin. Spectrophotometric observations are rationalized in terms of a static quenching process. The binding constants and the number of binding sites have been evaluated by the data obtained from the fluorescence quenching experiments. According to the vant Hoff equation, the standard enthalpy change (ΔH°) and standard entropy change (ΔS°) for the reaction were calculated and it indicated that the hydrophobic interactions play a main role in the binding of PUFX and pepsin. In addition, the binding distance R between pepsin (donor) and PUFX (acceptor) was obtained on the basis of the Forsters resonance energy transfer theory. The results obtained herein show that the interaction between the pepsin and PUFX was not influenced by Eu(III).
Keywords: PUFX, Pepsin, Fluorescence quenching, Binding constant, Eu(III), Binding Interaction, Spectroscopic Analysis, van, ’, t Hoff equation, Standard enthalpy change, Standard entropy change, Förster, s resonance energy transfer theory
Current Analytical Chemistry
Title: Analysis of Binding Interaction Between Prulifloxacin and Pepsin in the Existence of Eu(III): A Spectroscopic Analysis
Volume: 7 Issue: 3
Author(s): Xiaoyan Gao, Xiaojie Zhang, Yingcai Tang, Xiaoping Zhang, Wujie Zhao and Yanqin Zi
Affiliation:
Keywords: PUFX, Pepsin, Fluorescence quenching, Binding constant, Eu(III), Binding Interaction, Spectroscopic Analysis, van, ’, t Hoff equation, Standard enthalpy change, Standard entropy change, Förster, s resonance energy transfer theory
Abstract: Interaction between prulifloxacin (PUFX) and pepsin was studied under a simulated physiological condition using fluorescence spectrophotometric techniques when the Eu(III) exists. The fluorescence emission intensity of pepsin was strongly quenched by the addition of prulifloxacin. Spectrophotometric observations are rationalized in terms of a static quenching process. The binding constants and the number of binding sites have been evaluated by the data obtained from the fluorescence quenching experiments. According to the vant Hoff equation, the standard enthalpy change (ΔH°) and standard entropy change (ΔS°) for the reaction were calculated and it indicated that the hydrophobic interactions play a main role in the binding of PUFX and pepsin. In addition, the binding distance R between pepsin (donor) and PUFX (acceptor) was obtained on the basis of the Forsters resonance energy transfer theory. The results obtained herein show that the interaction between the pepsin and PUFX was not influenced by Eu(III).
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Cite this article as:
Gao Xiaoyan, Zhang Xiaojie, Tang Yingcai, Zhang Xiaoping, Zhao Wujie and Zi Yanqin, Analysis of Binding Interaction Between Prulifloxacin and Pepsin in the Existence of Eu(III): A Spectroscopic Analysis, Current Analytical Chemistry 2011; 7 (3) . https://dx.doi.org/10.2174/1573411011107030194
DOI https://dx.doi.org/10.2174/1573411011107030194 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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