Studies on the Electrochemical Behavior of Eugenol and its Application Using a Flow – Through Chronoamperometric Sensor

Lai-Hao      Wang; Jhih-Cheng      Chen

Abstract

The electrochemical behaviors of eugenol [1-hydroxy-2-methoxyl-4-allylbenzene] on the gold and platinum electrodes were investigated in a Britton-Robinson buffer (pH 1.60-10.44), acetate buffer, phosphate buffer solutions (pH 2.11 and 3.67) and methanol or acetonitrile containing various supporting electrolytes. The detection cell consisted of a gold wire electrochemical signal obtained with a supporting electrolyte containing 60 % methanol-1.0 mM of potassium dihydrogen phosphate (pH 2.40) as the mobile phase. The limit of quantification of the method was found to be 0.02 ng for eugenol. When eugenol was determined in commercial essential oils and pharmaceutical products with the method proposed the results obtained were comparable with those obtained by high – performance liquid chromatography with ultraviolet detection.

Keywords: Chronoamperometric sensor, Eugenol, Pharmaceutical products, Electrochemical Behavior, Britton-Robinson buffer, Limit of quantification, High, –, performance liquid chromatography, Direct current polarographic (DCP), Differential pulse polarographic (DPP), Dropping mercury electrode (DME), Linear sweep voltammetry, Cyclic voltammograms (CV), Precision