Abstract
Counter-current chromatography (CCC) is a unique support-free liquid-liquid partition chromatography winning wide applications in the separation of various components from natural or synthetic mixtures. It has been one of the prime methods for isolating compounds from Traditional Chinese Medicines (TCM) and other comprehensive natural products. Although early CCC models produced a long-standing false image that CCC is a time-consuming technique, rapid and high-performance CCC devices and methods for high throughput analysis of natural mixtures have been advanced. For instances, multi-channel CCC, dual CCC, elution-extrusion CCC, and solvent simplification protocols can provide high throughput CCC analysis and produce high purity of compounds or large natural product libraries for drug discovery. This review summarizes the recent advancements of CCC in the high throughput analysis of natural product with an emphasis on the developments of instruments and methods.
Keywords: Counter-current chromatography, elution-extrusion, high throughput, multi-channel, natural products, parallel protocol, Traditional Chinese Medicine, natural product libraries, drug discovery, metabolome, liquid-liquid partition chromatography, thin-layer chromatography, flash chromatography, batch-throughput solid-phase extraction, liquid chromatography-evaporative light scattering detection-mass spectrometry, LC-ELSD-MS, supercritical fluid chromatography, nuclear magnetic resonance, centrifugal partition chromatography, horizontal flow-through coil planet centrifuge, toroidal CPC, multilayer CPC, eccentric multi-layer CPC, high-speed counter-current chromatography, Brackenridgea, zanguebarica, Ochnaceae, Tephrosia vogelii, eguminosae, Solidago canadensis, Suillus placidus, Trichosanthes kirilowii, ELUTION-EXTRUSION COUNTER-CURRENT CHROMATOGRAPHY, hexane-ethyl acetate-methanol-water
Combinatorial Chemistry & High Throughput Screening
Title: Counter-Current Chromatography for High Throughput Analysis of Natural Products
Volume: 13 Issue: 10
Author(s): Shihua Wu and Junling Liang
Affiliation:
Keywords: Counter-current chromatography, elution-extrusion, high throughput, multi-channel, natural products, parallel protocol, Traditional Chinese Medicine, natural product libraries, drug discovery, metabolome, liquid-liquid partition chromatography, thin-layer chromatography, flash chromatography, batch-throughput solid-phase extraction, liquid chromatography-evaporative light scattering detection-mass spectrometry, LC-ELSD-MS, supercritical fluid chromatography, nuclear magnetic resonance, centrifugal partition chromatography, horizontal flow-through coil planet centrifuge, toroidal CPC, multilayer CPC, eccentric multi-layer CPC, high-speed counter-current chromatography, Brackenridgea, zanguebarica, Ochnaceae, Tephrosia vogelii, eguminosae, Solidago canadensis, Suillus placidus, Trichosanthes kirilowii, ELUTION-EXTRUSION COUNTER-CURRENT CHROMATOGRAPHY, hexane-ethyl acetate-methanol-water
Abstract: Counter-current chromatography (CCC) is a unique support-free liquid-liquid partition chromatography winning wide applications in the separation of various components from natural or synthetic mixtures. It has been one of the prime methods for isolating compounds from Traditional Chinese Medicines (TCM) and other comprehensive natural products. Although early CCC models produced a long-standing false image that CCC is a time-consuming technique, rapid and high-performance CCC devices and methods for high throughput analysis of natural mixtures have been advanced. For instances, multi-channel CCC, dual CCC, elution-extrusion CCC, and solvent simplification protocols can provide high throughput CCC analysis and produce high purity of compounds or large natural product libraries for drug discovery. This review summarizes the recent advancements of CCC in the high throughput analysis of natural product with an emphasis on the developments of instruments and methods.
Export Options
About this article
Cite this article as:
Wu Shihua and Liang Junling, Counter-Current Chromatography for High Throughput Analysis of Natural Products, Combinatorial Chemistry & High Throughput Screening 2010; 13 (10) . https://dx.doi.org/10.2174/138620710793360248
DOI https://dx.doi.org/10.2174/138620710793360248 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
Call for Papers in Thematic Issues
Artificial Intelligence Methods for Biomedical, Biochemical and Bioinformatics Problems
Recently, a large number of technologies based on artificial intelligence have been developed and applied to solve a diverse range of problems in the areas of biomedical, biochemical and bioinformatics problems. By utilizing powerful computing resources and massive amounts of data, methods based on artificial intelligence can significantly improve the ...read more
Eco-friendly Agents for Biological Control of Pathogenic Diseases
The discovery of an alternative biological approach to disease management includes work on medicinal products derived from natural sources as a starting point for the development of eco-friendly agents for these diseases and the injuries they cause, as well as reducing human contact with hazardous chemicals and their residues. We ...read more
Emerging trends in diseases mechanisms, noble drug targets and therapeutic strategies: focus on immunological and inflammatory disorders
Recently infectious and inflammatory diseases have been a key concern worldwide due to tremendous morbidity and mortality world Wide. Recent, nCOVID-9 pandemic is a good example for the emerging infectious disease outbreak. The world is facing many emerging and re-emerging diseases out breaks at present however, there is huge lack ...read more
Exploring Spectral Graph Theory in Combinatorial Chemistry
Scope of the Thematic Issue: Combinatorial chemistry involves the synthesis and analysis of a large number of diverse compounds simultaneously. Traditional methods rely on brute force experimentation, which can be time-consuming and resource-intensive. Spectral Graph Theory, a branch of mathematics dealing with the properties of graphs in relation to the ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Biochemical Differentiation of Cholinesterases from Normal and Alzheimers Disease Cortex
Current Alzheimer Research Editorial: Thematic Issue-Topic–Diabetic Cardiovascular Disease–An Unmet Medical Need: Emerging Targets and Therapies-Introduction to the Special Issue
Cardiovascular & Hematological Agents in Medicinal Chemistry Systemic Sclerosis Associated Interstitial Lung Disease
Current Respiratory Medicine Reviews Circadian Rhythms of Endothelial Nitric Oxide Synthase and Toll-like Receptors 2 Production in Females with Rheumatoid Arthritis Depending on NOS3 Gene Polymorphism
Reviews on Recent Clinical Trials Meloxicam-loaded Phospholipid/solutol® HS15 Based Mixed Nanomicelles: Preparation, Characterization, and in vitro Antioxidant Activity
Pharmaceutical Nanotechnology Headache: One of the Most Common and Troublesome Adverse Reactions to Drugs
Current Drug Safety Pharmacogenomics and Sepsis-Induced Renal Failure: Effects of β 2-Adrenoceptor Function on the Course of Sepsis
Current Pharmacogenomics and Personalized Medicine The Anti-inflammatory Potential of Selected Plant-derived Compounds in Respiratory Diseases
Current Pharmaceutical Design Nanotherapy Targeting the Tumor Microenvironment
Current Cancer Drug Targets About the Guest Editors
Recent Patents on Inflammation & Allergy Drug Discovery Synthesis and Biological Evaluation of Some Novel Thieno[2,3-d] pyrimidine Derivatives as Potential Anti-inflammatory and Analgesic Agents
Medicinal Chemistry Cell Therapy for the Treatment of Chronic Ischemic Heart Disease
Current Pharmaceutical Design Virus-Specific Peptide Dependent NK Cell Cytotoxicity
Inflammation & Allergy - Drug Targets (Discontinued) Melanoma Adjuvant Treatment: Current Insight and Clinical Features
Current Cancer Drug Targets Neuronal and Glial Responses to Polyamines in the Ischemic Brain
Current Neurovascular Research Adult Neural Stem Cells: Response to Stroke Injury and Potential for Therapeutic Applications
Current Stem Cell Research & Therapy Use of the Non-Toxic Cryoprotectant Trehalose Enhances Recovery and Function of Fish Embryonic Stem Cells Following Cryogenic Storage
Current Stem Cell Research & Therapy Phycobilins and Phycobiliproteins Used in Food Industry and Medicine
Mini-Reviews in Medicinal Chemistry Spatial Organization of Genomes
Current Genomics Potential Therapeutic Strategies to Combat HCC
Current Molecular Pharmacology