Abstract
Sequential injection analysis (SIA) has been employed as the platform for the development of a high throughput automated assay for glutathione (GSH) in nutrition supplements. The proposed assay is based on the on-line formation of a UV-absorbing thioacrylate derivative (δmax = 285 nm) upon reaction of thiol with the new reagent ethylpropiolate (EP). This is the very first application of this reagent for the determination of GSH either in batch or continuous flow modes and the first SI method for the determination of GSH in pharmaceutical supplements. The various variables that typically affect such a protocol were studied, while thorough validation experiments according to established standards proved the reliability of the assay for the intended applications. As many as 100 sample injections were enabled per hour.
Keywords: High throughput assay, glutathione, sequential injection, ethyl propiolate, UV-spectrophotometry
Combinatorial Chemistry & High Throughput Screening
Title: High Throughput Automated Determination of Glutathione Based on the Formation of a UV-Absorbing Thioacrylate Derivative
Volume: 13 Issue: 6
Author(s): Constantinos K. Zacharis and Paraskevas D. Tzanavaras
Affiliation:
Keywords: High throughput assay, glutathione, sequential injection, ethyl propiolate, UV-spectrophotometry
Abstract: Sequential injection analysis (SIA) has been employed as the platform for the development of a high throughput automated assay for glutathione (GSH) in nutrition supplements. The proposed assay is based on the on-line formation of a UV-absorbing thioacrylate derivative (δmax = 285 nm) upon reaction of thiol with the new reagent ethylpropiolate (EP). This is the very first application of this reagent for the determination of GSH either in batch or continuous flow modes and the first SI method for the determination of GSH in pharmaceutical supplements. The various variables that typically affect such a protocol were studied, while thorough validation experiments according to established standards proved the reliability of the assay for the intended applications. As many as 100 sample injections were enabled per hour.
Export Options
About this article
Cite this article as:
K. Zacharis Constantinos and D. Tzanavaras Paraskevas, High Throughput Automated Determination of Glutathione Based on the Formation of a UV-Absorbing Thioacrylate Derivative, Combinatorial Chemistry & High Throughput Screening 2010; 13 (6) . https://dx.doi.org/10.2174/138620710791515923
DOI https://dx.doi.org/10.2174/138620710791515923 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
Call for Papers in Thematic Issues
Artificial Intelligence Methods for Biomedical, Biochemical and Bioinformatics Problems
Recently, a large number of technologies based on artificial intelligence have been developed and applied to solve a diverse range of problems in the areas of biomedical, biochemical and bioinformatics problems. By utilizing powerful computing resources and massive amounts of data, methods based on artificial intelligence can significantly improve the ...read more
Eco-friendly Agents for Biological Control of Pathogenic Diseases
The discovery of an alternative biological approach to disease management includes work on medicinal products derived from natural sources as a starting point for the development of eco-friendly agents for these diseases and the injuries they cause, as well as reducing human contact with hazardous chemicals and their residues. We ...read more
Emerging trends in diseases mechanisms, noble drug targets and therapeutic strategies: focus on immunological and inflammatory disorders
Recently infectious and inflammatory diseases have been a key concern worldwide due to tremendous morbidity and mortality world Wide. Recent, nCOVID-9 pandemic is a good example for the emerging infectious disease outbreak. The world is facing many emerging and re-emerging diseases out breaks at present however, there is huge lack ...read more
Exploring Spectral Graph Theory in Combinatorial Chemistry
Scope of the Thematic Issue: Combinatorial chemistry involves the synthesis and analysis of a large number of diverse compounds simultaneously. Traditional methods rely on brute force experimentation, which can be time-consuming and resource-intensive. Spectral Graph Theory, a branch of mathematics dealing with the properties of graphs in relation to the ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Drug Delivery Nanosystems in Glioblastoma Multiforme Treatment: Current State of the Art
Current Neuropharmacology Antioxidant and Antimicrobial Activities of Cinnamic Acid Derivatives
Mini-Reviews in Medicinal Chemistry Label-Free Electrochemical Immunosensor for the Determination of Fetoprotein Based on Core-Shell-Shell Nanocomposite Particles
Protein & Peptide Letters Are the Myokines the Mediators of Physical Activity-Induced Health Benefits?
Current Pharmaceutical Design Synergistic Extraction of Zn2+ from Aqueous Buffer Medium of Chloride Ions into 1- Phenyl -3- Methyl -4- Trichloro Acetyl Pyrazolone - 5 in Benzene and Hexane
Recent Patents on Materials Science Gene Expression Abnormalities in Thymoma
Current Pharmacogenomics Efficient Transfection of Phosphorothioate Oligodeoxyribonucleotides by lipofectamine2000 into Different Bacteria
Current Drug Delivery Synthesis and Antiproliferative Evaluation of Spirothiadiazolopyridazine Derivatives
Letters in Drug Design & Discovery Production and Characterization of a Novel Bioflocculant from Klebsiella sp.
Current Biotechnology Current Application of Lipid- and Surfactant-based Vesicles for Cosmeceuticals: A Review
Current Pharmaceutical Biotechnology Mitochondrial Functionality and Chemical Compound Action on Sperm Function
Current Medicinal Chemistry The Molecular Recognition Paradigm of Environmental Chemicals with Biomacromolecules
Current Protein & Peptide Science Calixarene HPLC Phases - Applications
Current Analytical Chemistry Novel In Situ Activity Assays for the Quantitative Molecular Analysis of Neurodegenerative Processes in the Retina
Current Medicinal Chemistry Adsorption and Removal of Sudan I, II from Organic Solutions by Oxidized Multiwalled Carbon Nanotubes
Current Nanoscience Green Synthesis of Silver Nanoparticles Capped with Natural Carbohydrates Using Ephedra intermedia
Nanoscience & Nanotechnology-Asia Review: Alternative Splicing (AS) of Genes As An Approach for Generating Protein Complexity
Current Genomics Database Searching in Mass Spectrometry Based Proteomics
Current Bioinformatics Application of Proteomics to Investigate Plant-Microbe Interactions
Current Proteomics Step by Step Method Development and Analytical Validation of L929 Bioassay for rTNF-α Potency Evaluation in Manufacturing
Current Pharmaceutical Analysis