Abstract
The present study was designed to examine the uptake, localization, and the cytotoxic effects of well-dispersed fluorescent porous silica nanoparticles (FITC-SiO2 NPs) in mouse neural stem cells (C 17.2, NSCs). NSCs were exposed to various concentrations of FITC-SiO2 NPs for different times and then the uptake and toxicity were assessed. Apoptotic cells were observed and analyzed by confocal microscopy and flow cytometry. Results of confocal microscopy examination revealed that silica nanoparticles were taken up into the cells. Cell viability decreased significantly as a function of both nanoparticle dosage (12- 240 μg/ml) and exposure time (12 h, 24 h 72 h). FITC-SiO2 NPs show marked toxicity at high concentration (240 μg/mL) after co-incubation for 72 h. There were clear dose- and timedependent silica-induced cytotoxicity and genotoxicity within the range of experimental concentrations. Interestingly, we have caught the whole process of NSCs apoptosis induced by FITC-SiO2 NPs. The understanding of such a mechanism may provide a scientific basis for the possible application of porous silica in drug delivery and controlled release.
Keywords: Fluorescence, Porous silica nanoparticles, Cytotoxicity, Apoptosis, Neural stem cell
Current Nanoscience
Title: Direct Imaging of Apoptosis Process of Neural Stem Cells Exposed to Porous Silica Nanoparticles
Volume: 6 Issue: 3
Author(s): Yanli Wang, Shun Tan, Jia Wang, Qiuxia Wu, Xinxin Chen, Xiaoyong Deng, Qiong Lu and Minghong Wu
Affiliation:
Keywords: Fluorescence, Porous silica nanoparticles, Cytotoxicity, Apoptosis, Neural stem cell
Abstract: The present study was designed to examine the uptake, localization, and the cytotoxic effects of well-dispersed fluorescent porous silica nanoparticles (FITC-SiO2 NPs) in mouse neural stem cells (C 17.2, NSCs). NSCs were exposed to various concentrations of FITC-SiO2 NPs for different times and then the uptake and toxicity were assessed. Apoptotic cells were observed and analyzed by confocal microscopy and flow cytometry. Results of confocal microscopy examination revealed that silica nanoparticles were taken up into the cells. Cell viability decreased significantly as a function of both nanoparticle dosage (12- 240 μg/ml) and exposure time (12 h, 24 h 72 h). FITC-SiO2 NPs show marked toxicity at high concentration (240 μg/mL) after co-incubation for 72 h. There were clear dose- and timedependent silica-induced cytotoxicity and genotoxicity within the range of experimental concentrations. Interestingly, we have caught the whole process of NSCs apoptosis induced by FITC-SiO2 NPs. The understanding of such a mechanism may provide a scientific basis for the possible application of porous silica in drug delivery and controlled release.
Export Options
About this article
Cite this article as:
Wang Yanli, Tan Shun, Wang Jia, Wu Qiuxia, Chen Xinxin, Deng Xiaoyong, Lu Qiong and Wu Minghong, Direct Imaging of Apoptosis Process of Neural Stem Cells Exposed to Porous Silica Nanoparticles, Current Nanoscience 2010; 6 (3) . https://dx.doi.org/10.2174/157341310791171207
DOI https://dx.doi.org/10.2174/157341310791171207 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
Call for Papers in Thematic Issues
Role of nanomaterials in fabrication of coatings, Machining and Joining
The application of nanoscience has brought about a revolution in the field of mechanical engineering by providing novel materials, boosting manufacturing processes, and generating cutting-edge products. The purpose of this special issue is to investigate the enormous impact that nanoscience has had on mechanical engineering, with a particular emphasis on ...read more
Advanced Inorganic Nanocomposites and Their Emerging Applications
This special issue collection will highlight developments on the recent trends about the synthesis of metal oxides, nanoclusters, biomaterials, 2D nanomaterials, nanocrystals, nanocomposites, etc. and their applications in electrochemical systems, tissue regeneration, energy storage and harvesting, sensors, etc. The novelty of the methods in the chemical synthesis and their characterizations, ...read more
Applicability of Nanotechnology for Performance Enhancement of Clean Energy Technologies
Population growth, industrialization, and improvement in living quality would lead to further increase in energy demand in near future. Regarding the disadvantages of fossil fuels such as fluctuations in their price, high emissions of greenhouse gases and restriction of their sources, it is crucial to use and exploit alternative energy ...read more
Graphene and 2D Materials for Energy Storage and Conversion
This thematic issue will discuss the recent advances in graphene-based nanomaterials for different energy technologies. Graphene possesses a high surface area, and stable structure and exhibits many interesting electronic, optical, and mechanical properties due to its 2D crystal structure. Graphene is of both fundamental interest and suitable for a wide ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Lung Cancer- Current Clinical Profile and Diagnostic Challenges
Current Respiratory Medicine Reviews The Impact of DIDS-Induced Inhibition of Voltage-Dependent Anion Channels (VDAC) on Cellular Response of Lymphoblastoid Cells to Ionizing Radiation
Medicinal Chemistry Anticancer Effects of Ginsenoside Rh2: A Systematic Review
Current Molecular Pharmacology Implications of FLT3 Mutations in the Therapy of Acute Myeloid Leukemia
Reviews on Recent Clinical Trials ABC Transporters: Regulation and Association with Multidrug Resistance in Hepatocellular Carcinoma and Colorectal Carcinoma
Current Medicinal Chemistry Genetic Instability Influences Drug Response in Cancer Cells
Current Drug Targets Angiogenesis and Angiogenesis Inhibitors: a New Potential Anticancer Therapeutic Strategy
Current Drug Targets - Immune, Endocrine & Metabolic Disorders Fragment-Based Optimization of Small Molecule CXCL12 Inhibitors for Antagonizing the CXCL12/CXCR4 Interaction
Current Topics in Medicinal Chemistry Novel Rational Drug Design Strategies with Potential to Revolutionize Malaria Chemotherapy
Current Medicinal Chemistry Identification of Novel Drug Targets for Angiostatic Cancer Therapy; It Takes Two to Tango
Current Pharmaceutical Design Betulinic Acid Kills Colon Cancer Stem Cells
Current Stem Cell Research & Therapy Eco-friendly Synthesis of Pyrido[2,3-d]pyrimidine Analogs and Their Anticancer and Tyrosine Kinase Inhibition Activities
Anti-Cancer Agents in Medicinal Chemistry Organoselenium Compounds in Cancer Chemoprevention
Mini-Reviews in Medicinal Chemistry Ribozyme- and Deoxyribozyme-Strategies for Medical Applications
Current Drug Targets Synthesis and Biological Activity of Akt/PI3K Inhibitors
Mini-Reviews in Medicinal Chemistry Personalized Therapies in Pediatric Inflammatory and Autoimmune Diseases
Current Pharmaceutical Design Adhesion Dependent Signalling in the Tumour Microenvironment: The Future of Drug Targetting
Current Pharmaceutical Design Silencing GPX3 Expression Promotes Tumor Metastasis in Human Thyroid Cancer
Current Protein & Peptide Science Recent Advances in Molecular Modeling and Medicinal Chemistry Aspects of Phospho-Glycoprotein&#
Current Drug Metabolism Do We have the Evidence to Repurpose Levamisole for Treatment of COVID 19?
Current Drug Therapy