Abstract
The investigation of nano-mechanical properties and nano-architectures of CD8 + T cells activated in vitro will help further interpret the immune response/recognition of these cells at nanoscale resolution. In this work, the local biophysical properties of human peripheral blood CD8 + T cells were obtained, revealing the adhesion force of membrane was increased after CD8 + T cells were stimulated with phorbol dibutyrate plus ionomycin, though the measured local stiffness for resting and activated cells was similar. The 3-D distribution pattern and grayscale map together revealed that membrane receptors (CD8 and activation marker CD69) distributed non-uniformly on the membrane and were shown as micro-, nano-scale clusters or domains. This work describes a relatively simple approach for exploiting the distribution pattern of receptor molecules on a single T cell surface.
Keywords: Force spectroscopy, molecule recognition, receptor mapping, nano-mechanics, CD8 + T cell
Current Nanoscience
Title: Nano-Mechanics and Membrane Receptor Mapping of CD8 + T Cells (Supplementry Material)
Volume: 5 Issue: 4
Author(s): Yangzhe Wu, Yi Hu, Hongsong Lu, Jiye Cai, Xianhui He and Hongxia Zhao
Affiliation:
Keywords: Force spectroscopy, molecule recognition, receptor mapping, nano-mechanics, CD8 + T cell
Abstract: The investigation of nano-mechanical properties and nano-architectures of CD8 + T cells activated in vitro will help further interpret the immune response/recognition of these cells at nanoscale resolution. In this work, the local biophysical properties of human peripheral blood CD8 + T cells were obtained, revealing the adhesion force of membrane was increased after CD8 + T cells were stimulated with phorbol dibutyrate plus ionomycin, though the measured local stiffness for resting and activated cells was similar. The 3-D distribution pattern and grayscale map together revealed that membrane receptors (CD8 and activation marker CD69) distributed non-uniformly on the membrane and were shown as micro-, nano-scale clusters or domains. This work describes a relatively simple approach for exploiting the distribution pattern of receptor molecules on a single T cell surface.
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Cite this article as:
Wu Yangzhe, Hu Yi, Lu Hongsong, Cai Jiye, He Xianhui and Zhao Hongxia, Nano-Mechanics and Membrane Receptor Mapping of CD8 + T Cells (Supplementry Material), Current Nanoscience 2009; 5 (4) . https://dx.doi.org/10.2174/157341309789378096
DOI https://dx.doi.org/10.2174/157341309789378096 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
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