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Current Genomics

Editor-in-Chief

ISSN (Print): 1389-2029
ISSN (Online): 1875-5488

TWIST1 Gene: First Insights in Felis catus

Author(s): Claudia S. Baptista, Estela Bastos, Sara Santos, Ivo G. Gut, Henrique Guedes-Pinto, Fatima Gartner and Raquel Chaves

Volume 11, Issue 3, 2010

Page: [212 - 220] Pages: 9

DOI: 10.2174/138920210791110933

Price: $65

Abstract

TWIST1 is thought to be a novel oncogene. Understanding the molecular mechanisms regulating the TWIST1 gene expression profiles in tumor cells may give new insights regarding prognostic factors and novel therapeutic targets in veterinary oncology. In the present study we partially isolated the TWIST1 gene in Felis catus and performed comparative studies. Several primer combinations were used based on the alignments of homologous DNA sequences. After PCR amplification, three bands were obtained, purified and sequenced. Several bioinformatic tools were utilized to carry out the comparative studies. Higher similarity was found between the isolated TWIST1 gene in Felis catus and Homo sapiens (86%) than between Homo sapiens and Rattus norvegicus or Mus musculus (75%). Partial amino acid sequence showed no change in the four species analyzed. This confirmed that coding sequences presented high similarity (∼96%) between man and cat. These results give the first insights regarding the TWIST1 gene in cat but further studies are required in order to establish, or not, its role in tumor formation and progression in veterinary oncology.

Keywords: TWIST1 gene, Felis catus, comparative analysis, oncology

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