Abstract
The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without the use of labels. Advances in biosensor design and detection using BioLayer Interferometry (BLI) provide a simple platform that enables label-free monitoring of biomolecular interactions without the use of flow cells. We review the applications of BLI in a wide variety of research and development environments for quantifying antibodies and proteins and measuring kinetics parameters.
Combinatorial Chemistry & High Throughput Screening
Title: Label-Free Detection of Biomolecular Interactions Using BioLayer Interferometry for Kinetic Characterization
Volume: 12 Issue: 8
Author(s): Donald Perillat, Hong Tan, Robert Zuk, Scott Lockard, Janette Phi-Wilson, Michael Howes, Juan Gamez, Tim Ellis, Huddee Ho, Kevin Du, Michael Recknor, Greg Carricato, Joy Concepcion, Lian-She Zhao, Ram Varma, Weilei Ma, Bettina Heidecker, Pu Li, Jing Wei, Henrik Persson, Danfeng Yao, Sae Choo, Charles Wartchow and Krista Witte
Affiliation:
Abstract: The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without the use of labels. Advances in biosensor design and detection using BioLayer Interferometry (BLI) provide a simple platform that enables label-free monitoring of biomolecular interactions without the use of flow cells. We review the applications of BLI in a wide variety of research and development environments for quantifying antibodies and proteins and measuring kinetics parameters.
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Perillat Donald, Tan Hong, Zuk Robert, Lockard Scott, Phi-Wilson Janette, Howes Michael, Gamez Juan, Ellis Tim, Ho Huddee, Du Kevin, Recknor Michael, Carricato Greg, Concepcion Joy, Zhao Lian-She, Varma Ram, Ma Weilei, Heidecker Bettina, Li Pu, Wei Jing, Persson Henrik, Yao Danfeng, Choo Sae, Wartchow Charles and Witte Krista, Label-Free Detection of Biomolecular Interactions Using BioLayer Interferometry for Kinetic Characterization, Combinatorial Chemistry & High Throughput Screening 2009; 12 (8) . https://dx.doi.org/10.2174/138620709789104915
DOI https://dx.doi.org/10.2174/138620709789104915 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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