Abstract
In this contribution, the rapid and simple determination of vitamins A and E, and vitamin E acetate as internal standard in human plasma by high-performance liquid chromatography with a column-switching technique was proposed. Restricted Access Material - RAM column 25 x 4 mm containing 25 μm C18 alkyl-diol silica support was integrated into lab-made column-switching HPLC system. A monolithic column Chromolith Performance RP-18e, 100 x 4.6 mm, (Merck) was used for simple separation of vitamins in gradient mode. The mobile phase I: methanol – 20 mM water solution of dodecyl sulfate sodium salt (30:70, v/v) was used for protein matrix elution, flow rate 1.3 ml min-1. Valve switching time was determined to 4 minutes after sample injection. The gradient elution of mobile phase II: methanol – water 90:10 (v/v) → 100% methanol, flow rate 1.0 ml min-1 was used as optimal condition of vitamins separation in relatively short time. Analysis time from raw sample to peaks evaluation was less than 15 min. UV detection was carried out at wavelength 285 nm. The recoveries of vitamins from spiked human plasma were 102.5% for vitamin A and 91.4% for vitamin E. Limits of detection were 0.24 µmol l-1 and 0.84 μmol l-1 for vitamin A and E, respectively.
Keywords: Vitamin A, Vitamin E, Vitamin E acetate, Column-switching, Restricted Access Material (RAM), HPLC
Current Analytical Chemistry
Title: Rapid and Simple Determination of Vitamin A and Vitamin E in Human Plasma by Column-Switching High-Performance Liquid Chromatography
Volume: 5 Issue: 4
Author(s): Petr Sadilek, Dalibor Satinsky, Martin Otapka, Radek Sladkovsky and Petr Solich
Affiliation:
Keywords: Vitamin A, Vitamin E, Vitamin E acetate, Column-switching, Restricted Access Material (RAM), HPLC
Abstract: In this contribution, the rapid and simple determination of vitamins A and E, and vitamin E acetate as internal standard in human plasma by high-performance liquid chromatography with a column-switching technique was proposed. Restricted Access Material - RAM column 25 x 4 mm containing 25 μm C18 alkyl-diol silica support was integrated into lab-made column-switching HPLC system. A monolithic column Chromolith Performance RP-18e, 100 x 4.6 mm, (Merck) was used for simple separation of vitamins in gradient mode. The mobile phase I: methanol – 20 mM water solution of dodecyl sulfate sodium salt (30:70, v/v) was used for protein matrix elution, flow rate 1.3 ml min-1. Valve switching time was determined to 4 minutes after sample injection. The gradient elution of mobile phase II: methanol – water 90:10 (v/v) → 100% methanol, flow rate 1.0 ml min-1 was used as optimal condition of vitamins separation in relatively short time. Analysis time from raw sample to peaks evaluation was less than 15 min. UV detection was carried out at wavelength 285 nm. The recoveries of vitamins from spiked human plasma were 102.5% for vitamin A and 91.4% for vitamin E. Limits of detection were 0.24 µmol l-1 and 0.84 μmol l-1 for vitamin A and E, respectively.
Export Options
About this article
Cite this article as:
Sadilek Petr, Satinsky Dalibor, Otapka Martin, Sladkovsky Radek and Solich Petr, Rapid and Simple Determination of Vitamin A and Vitamin E in Human Plasma by Column-Switching High-Performance Liquid Chromatography, Current Analytical Chemistry 2009; 5 (4) . https://dx.doi.org/10.2174/157341109789077722
DOI https://dx.doi.org/10.2174/157341109789077722 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
New Approaches for the Synthesis of Heterocyclic Compounds Corporating Benzo[d]imidazole as Anticancer Agents, Tyrosine, Pim-1 Kinases Inhibitions and their PAINS Evaluations
Anti-Cancer Agents in Medicinal Chemistry Redox-Sensitive Smart Nanosystems for Drug and Gene Delivery
Current Organic Chemistry Analgesic and Antineuropathic Drugs Acting Through Central Cholinergic Mechanisms
Recent Patents on CNS Drug Discovery (Discontinued) Intrinsic Disorder-Based Design of Stabilizing Disulphide Bridge in Gαo Protein
Protein & Peptide Letters Use of High-Throughput Trypsin Digestion in Proteomic Studies
Current Proteomics Target Based Virtual Screening of New Leads Inhibitor against Bacterial Cell Division Protein FtsZ for the Discovery of Antibacterial Agents
Medicinal Chemistry Structure Function Relationships of KTS Disintegrins and Design of Antiangiogenic Drugs
Letters in Drug Design & Discovery Acyclonucleosides, Modified Seco-Nucleosides, and Salicyl- or Catechol- Derived Acyclic 5-Fluorouracil O,N-Acetals: Antiproliferative Activities, Cellular Differentiation and Apoptosis
Current Medicinal Chemistry Quantitative Proteomics with Isotope Dilution Analysis: Principles and Applications
Current Proteomics Decision Support System for Lymphoma Classification
Current Medical Imaging Aptamers in Drug Design: An Emerging Weapon to Fight a Losing Battle
Current Drug Targets Advances in Antioxidant Potential of Natural Alkaloids
Current Bioactive Compounds Determination of Dysregulated miRNA Expression Levels by qRT-PCR after the Application of Usnic Acid to Breast Cancer
Anti-Cancer Agents in Medicinal Chemistry Characterization of the Lipid Soluble Fraction of Brewer's Spent Grain
Current Chromatography Microarrays and Mass Spectrometry - The Future of Proteomics
Current Genomics Insights on Antimicrobial Resistance, Biofilms and the Use of Phytochemicals as New Antimicrobial Agents
Current Medicinal Chemistry 3,4-Dihydrospiro[piperidine-4,2-(1H)quinoline] Derivatives as New Antioxidant Agents with Acetylcholinesterase Inhibitory Property
Letters in Drug Design & Discovery The Role of Alpha-lipoic Acid Supplementation in the Prevention of Diabetes Complications: A Comprehensive Review of Clinical Trials
Current Diabetes Reviews Metformin Protects the Brain Against the Oxidative Imbalance Promoted by Type 2 Diabetes
Medicinal Chemistry Knee Osteoarthritis and Exercise Adherence: A Review
Current Aging Science