Abstract
In this work, water-compatible molecularly imprinted polymers (MIPs) were prepared for fast, accurate and selective solid-phase extraction of verapamil (VPM) from complex matrices such as biological fluids and human urine followed by its UV spectroscopic determination at 278 nm. The effective factors influencing the precipitation polymerization have been studied. Molecular recognition properties, binding capability and selectivity of the MIPs were evaluated and the results revealed that the obtained MIPs have high affinity for VPM in aqueous media. Equilibrium binding experiments were done to assess the performance of the MIP relative to non imprinted polymer (NIP). After optimization of molecularly imprinted solid-phase extraction (MISPE) method with 2 mL water plus 2 mL acetone as washing solvents and 5 mL of methanol and acetic acid (10:1, v/v) as elution solvent, successful imprinting was confirmed by comparison of the recoveries between NIP (4%) and MIP (97%) polymers. The binding capacity of the MIP for VPM was determined to be 196 mg g-1 (400 μmol g-1). Accuracy and precision were checked by the HPLC technique and the results did not present significant difference at 95% confidence levels according to the t-test.
Keywords: Molecularly imprinted polymer, Solid-phase extraction, Verapamil, Biological fluids, Human urine
Current Pharmaceutical Analysis
Title: Molecularly Imprinted Polymers for Selective Solid-Phase Extraction of Verapamil from Biological Fluids and Human Urine
Volume: 5 Issue: 3
Author(s): Mehran Javanbakht, Narges Shaabani, Majid Abdouss, Mohammad R. Ganjali, Ali Mohammadi and Parviz Norouzi
Affiliation:
Keywords: Molecularly imprinted polymer, Solid-phase extraction, Verapamil, Biological fluids, Human urine
Abstract: In this work, water-compatible molecularly imprinted polymers (MIPs) were prepared for fast, accurate and selective solid-phase extraction of verapamil (VPM) from complex matrices such as biological fluids and human urine followed by its UV spectroscopic determination at 278 nm. The effective factors influencing the precipitation polymerization have been studied. Molecular recognition properties, binding capability and selectivity of the MIPs were evaluated and the results revealed that the obtained MIPs have high affinity for VPM in aqueous media. Equilibrium binding experiments were done to assess the performance of the MIP relative to non imprinted polymer (NIP). After optimization of molecularly imprinted solid-phase extraction (MISPE) method with 2 mL water plus 2 mL acetone as washing solvents and 5 mL of methanol and acetic acid (10:1, v/v) as elution solvent, successful imprinting was confirmed by comparison of the recoveries between NIP (4%) and MIP (97%) polymers. The binding capacity of the MIP for VPM was determined to be 196 mg g-1 (400 μmol g-1). Accuracy and precision were checked by the HPLC technique and the results did not present significant difference at 95% confidence levels according to the t-test.
Export Options
About this article
Cite this article as:
Javanbakht Mehran, Shaabani Narges, Abdouss Majid, Ganjali R. Mohammad, Mohammadi Ali and Norouzi Parviz, Molecularly Imprinted Polymers for Selective Solid-Phase Extraction of Verapamil from Biological Fluids and Human Urine, Current Pharmaceutical Analysis 2009; 5 (3) . https://dx.doi.org/10.2174/157341209788922011
DOI https://dx.doi.org/10.2174/157341209788922011 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Ebola Virus Altered Innate and Adaptive Immune Response Signalling Pathways: Implications for Novel Therapeutic Approaches
Infectious Disorders - Drug Targets Role of Infrared Spectroscopy in Medicinal Plants Research in Pakistan
Current Bioactive Compounds The Heart in Sepsis: From Basic Mechanisms to Clinical Management
Current Vascular Pharmacology Cyclin Dependent Kinase 1 Inhibitors: A Review of Recent Progress
Current Medicinal Chemistry Improvement of Nerve Fiber Density in Fibromyalgia Patients Treated with IVIg
Current Rheumatology Reviews Therapeutic Interventions in the Glyc(oxid)ation Pathway
Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Discontinued) Noradrenaline in Parkinsons Disease: From Disease Progression to Current Therapeutics
Current Medicinal Chemistry Challenges in the Management of Paediatric Febrile Neutropenia
Current Pediatric Reviews Diagnosis and Management of Heart Failure with Preserved Ejection Fraction: 10 Key Lessons
Current Cardiology Reviews Noradrenergic Regulation of Glial Activation: Molecular Mechanisms and Therapeutic Implications
Current Neuropharmacology Ranolazine : Effects on Ischemic Heart
Recent Patents on Cardiovascular Drug Discovery Ischemic and Oxidative Damage to the Hypothalamus May Be Responsible for Heat Stroke
Current Neuropharmacology Drug Delivery to CNS: Challenges and Opportunities with Emphasis on Biomaterials Based Drug Delivery Strategies
Current Pharmaceutical Design Pegfilgrastim-Apgf (Nyvepria): Biosimilar USFDA Approval for the Treatment of Chemotherapy-induced Febrile Neutropenia and Current Updates on Clinical Trials
Current Drug Targets Diabetic Gastroenteropathy: Soothe the Symptoms or Unravel a Cure?
Current Diabetes Reviews Contemporary Treatment of Amyloid Heart Disease
Current Pharmaceutical Design From the Oxygen to the Organ Protection: Erythropoietin as Protagonist in Internal Medicine
Cardiovascular & Hematological Agents in Medicinal Chemistry Use of Corticosteroids in Critically Ill Septic Patients: A Review of Mechanisms of Adrenal Insufficiency in Sepsis and Treatment
Current Drug Targets Amlodipine (150 mg) Poisoning: A Case Study
Current Drug Safety Ribonucleases, Nucleases and Antiangiogenins in Antiproliferative Activities
Current Signal Transduction Therapy